Nagahara Rei, Kimura Masayuki, Itahashi Megu, Sugahara Go, Kawashima Masashi, Murayama Hirotada, Yoshida Toshinori, Shibutani Makoto
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, Tokyo, Japan (Nagahara, Kimura, Itahashi, Kawashima, Murayama, Yoshida, Shibutani)Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan (Sugahara).
Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, Tokyo, Japan (Nagahara, Kimura, Itahashi, Kawashima, Murayama, Yoshida, Shibutani)Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan (Sugahara)
J Vet Diagn Invest. 2016 Nov;28(6):722-728. doi: 10.1177/1040638716664381. Epub 2016 Oct 3.
Two solitary and minute tumors of 1 and 1.5 mm diameter were identified by microscopy in the left fourth mammary gland of a 13-year-old female Labrador Retriever dog, in addition to multiple mammary gland tumors. The former tumors were well circumscribed and were composed of small-to-large polyhedral neoplastic oncocytes with finely granular eosinophilic cytoplasm, and were arranged in solid nests separated by fine fibrovascular septa. Scattered lumina of variable sizes containing eosinophilic secretory material were evident. Cellular atypia was minimal, and no mitotic figures were visible. One tumor had several oncocytic cellular foci revealing cellular transition, with perivascular pseudorosettes consisting of columnar epithelial cells surrounding the fine vasculature. Scattered foci of mammary acinar cell hyperplasia showing oncocytic metaplasia were also observed. Immunohistochemically, the cytoplasm of neoplastic cells of the 2 microtumors showed diffuse immunoreactivity to anti-cytokeratin antibody AE1/AE3, and finely granular immunoreactivity for 60-kDa heat shock protein, mitochondrial membrane ATP synthase complex V beta subunit, and chromogranin A. One tumor also had oncocytic cellular foci forming perivascular pseudorosettes showing cellular membrane immunoreactivity for neural cell adhesion molecule. The tumors were negative for smooth muscle actin, neuron-specific enolase, vimentin, desmin, S100, and synaptophysin. Ultrastructural observation confirmed the abundant mitochondria in the cytoplasm of both neoplastic and hyperplastic cells, the former cells also having neuroendocrine granule-like electron-dense bodies. From these results, our case was diagnosed with mammary oncocytomas accompanied by neuroendocrine differentiation. Scattered foci of mammary oncocytosis might be related to the multicentric occurrence of these oncocytomas.
在一只13岁雌性拉布拉多寻回犬的左第四乳腺中，显微镜检查发现除了多个乳腺肿瘤外，还有两个直径分别为1毫米和1.5毫米的孤立微小肿瘤。前一种肿瘤边界清晰，由小到大的多面体肿瘤性嗜酸性细胞组成，细胞质呈细颗粒状嗜酸性，排列成由细纤维血管间隔分隔的实性巢状结构。可见大小不一的散在管腔，内含嗜酸性分泌物质。细胞异型性极小，未见有丝分裂象。一个肿瘤有几个嗜酸性细胞灶显示细胞过渡，有由围绕细血管的柱状上皮细胞组成的血管周围假菊形团。还观察到散在的乳腺腺泡细胞增生灶，显示嗜酸性化生。免疫组织化学检查显示，这两个微肿瘤的肿瘤细胞胞质对抗细胞角蛋白抗体AE1/AE3呈弥漫性免疫反应，对60-kDa热休克蛋白、线粒体膜ATP合酶复合物Vβ亚基和嗜铬粒蛋白A呈细颗粒状免疫反应。一个肿瘤的嗜酸性细胞灶还形成血管周围假菊形团，对神经细胞黏附分子呈细胞膜免疫反应。肿瘤对平滑肌肌动蛋白、神经元特异性烯醇化酶、波形蛋白、结蛋白、S100和突触素均为阴性。超微结构观察证实，肿瘤细胞和增生细胞的胞质内均有丰富的线粒体，前者细胞还具有神经内分泌颗粒样电子致密体。根据这些结果，本病例诊断为伴有神经内分泌分化的乳腺嗜酸性细胞瘤。散在的乳腺嗜酸性细胞灶可能与这些嗜酸性细胞瘤的多中心发生有关。
