Department of Biology, University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada.
London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.
Sci Rep. 2017 Jan 11;7:39550. doi: 10.1038/srep39550.
Cyclophilins (CYPs) belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. They catalyze the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. A yeast-two-hybrid screening using the isoflavonoid regulator GmMYB176 as bait identified GmCYP1 as one of the interacting proteins in soybean embryos. GmCYP1 localizes both in the nucleus and cytoplasm, and interacts in planta with GmMYB176, in the nucleus, and with SGF14l (a soybean 14-3-3 protein) in the nucleus and the cytoplasm. GmCYP1 contains a single cyclophilin-like domain and displays a high sequence identity with other plant CYPs that are known to have stress-specific function. Tissue-specific expression of GmCYP1 revealed higher expression in developing seeds compared to other vegetative tissues, suggesting their seed-specific role. Furthermore, GmCYP1 transcript level was reduced in response to stress. Since isoflavonoids are involved in plant stress resistance against biotic and abiotic factors, the interaction of GmCYP1 with the isoflavonoid regulators GmMYB176 and 14-3-3 protein suggests its role in defense in soybean.
细胞色素 P450（CYPs）属于免疫亲和素超家族，具有肽基脯氨酰顺反异构酶（PPIase）活性。它们催化肽基脯氨酰酰胺键的顺式和反式异构体的互变。使用异黄酮调节剂 GmMYB176 作为诱饵的酵母双杂交筛选鉴定了 GmCYP1 是大豆胚胎中相互作用蛋白之一。GmCYP1 定位于细胞核和细胞质中，并且在体内与 GmMYB176 在细胞核中，与 SGF14l（一种大豆 14-3-3 蛋白）在细胞核和细胞质中相互作用。GmCYP1 含有单个细胞色素 P450 样结构域，与其他已知具有应激特异性功能的植物细胞色素 P450 具有高度的序列同一性。GmCYP1 的组织特异性表达显示在发育中的种子中比其他营养组织表达更高，表明其在种子中的特异性作用。此外，GmCYP1 的转录水平在受到胁迫时降低。由于异黄酮参与植物对生物和非生物因素的应激抗性，GmCYP1 与异黄酮调节剂 GmMYB176 和 14-3-3 蛋白的相互作用表明其在大豆防御中的作用。
