Department of Materials Science & Engineering, North Carolina State University, Raleigh, North Carolina 27606, USA.
Nanoscale. 2017 Jan 26;9(4):1637-1644. doi: 10.1039/c6nr08433h.
Structural DNA nanotechnology has demonstrated both versatility and potential as a molecular manufacturing tool; the formation and processing of DNA nanostructures has therefore been subject to much interest. Characterization of the formation process itself is vital to understanding the role of design in production yield. We present our search for a robust new technique, chemical quenching, to arrest molecular folding in DNA systems for subsequent characterization. Toward this end we will introduce two miniM13 origami designs based on a 2.4 kb scaffold, each with diametrically opposed scaffold routing strategies (maximized scaffold crossovers versus maximized staple crossovers) to examine the relevance of design in the folding process. By chemically rendering single strand DNA inert and unable to hybridize, we probe the folding pathway of several scaffolded DNA origami structures.
结构 DNA 纳米技术已被证明具有多功能性和作为分子制造工具的潜力；因此，DNA 纳米结构的形成和处理引起了广泛关注。对形成过程本身的特征分析对于理解设计在产量中的作用至关重要。我们正在寻找一种新的稳健技术——化学淬灭，以在 DNA 系统中阻止分子折叠，以便随后进行特征分析。为此，我们将介绍两个基于 2.4 kb 支架的 miniM13 折纸设计，每个设计都采用直径相反的支架布线策略（最大化支架交叉点与最大化订书钉交叉点），以研究设计在折叠过程中的相关性。通过使单链 DNA 失活且无法杂交，我们探测了几个支架 DNA 折纸结构的折叠途径。
