National Institute of Standards and Technology, Gaithersburg, Maryland 20899-6203, United States.
ACS Nano. 2021 Feb 23;15(2):3284-3294. doi: 10.1021/acsnano.0c10114. Epub 2021 Feb 10.
Understanding the folding process of DNA origami is a critical stepping stone to the broader implementation of nucleic acid nanofabrication technology but is notably nontrivial. Origami are formed by several hundred cooperative hybridization events-folds-between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples. Individual events are difficult to detect. Here, we present a real-time probe of the unit operation of origami assembly, a single fold, across the scaffold as a function of hybridization domain separation-fold distance-and staple/scaffold ratio. This approach to the folding problem elucidates a predicted but previously unobserved blocked state that acts as a limit on yield for single folds, which may manifest as a barrier in whole origami assembly.
理解 DNA 折纸的折叠过程是广泛实施核酸纳米制造技术的关键步骤，但这并不简单。折纸由来自病毒基因组的支架和寡核苷酸订书钉的几个数百个协同杂交事件折叠形成。单个事件很难检测到。在这里，我们提出了一种实时探测折纸组装的基本操作的方法，即作为杂交域分离折叠距离和订书钉/支架比的函数的单个折叠跨越支架。这种折纸折叠问题的研究方法阐明了一种预测但以前未观察到的受阻状态，该状态作为单折叠产率的限制因素，这可能在整个折纸组装中表现为障碍。
