Chen Zhung-Yuan, Hsieh You-Miin, Huang Chun-Chih, Tsai Cheng-Chih
Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan.
New Bellus Enterprises Co., Ltd. No. 48, Industrial Rd., Erh Chen Vil., Kuan Tien Dist., Tainan City 72042, Taiwan.
Molecules. 2017 Jan 10;22(1):107. doi: 10.3390/molecules22010107.
This study was conducted to investigate the inhibitory effect of cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1.19, which showed a significant difference from the other control and LAB groups ( < 0.05), which therefore led to the inference that the BCRC17010 strain exerts a pro-apoptotic effect on the HT-29 cells. Upon co-culture with HT-29 cells for 4, 8 and 12 h, the BCRC14625 strain (10⁸ cfu/mL) demonstrated a significant increase in lactate dehydrogenase (LDH) activity ( < 0.05), causing harm to the HT-29 cell membrane; further, after an 8-h co-culture with the HT-29 cells, it induced the secretion of nitric oxide (NO) from the HT-29 cells. Some lactic acid bacteria (LAB) strains have ability to inhibit the growth of the colorectal cancer cell line HT-29 Bax/Bcl-2 pathway or NO production. In summary, we demonstrated that the BCRC17010 strain, good abilities of adhesion and increased LDH release, was the best probiotic potential for inhibition of HT-29 growth amongst the seven LAB strains tested in vitro.
本研究旨在探讨细胞及上清液对人结肠癌细胞系HT - 29生长的抑制作用。我们的研究结果表明，PM153菌株在胃肠道模拟实验中表现出最佳的黏附能力和最高的存活率。此外，PM153与HT - 29细胞共培养8小时后，PM153菌株可诱导HT - 29细胞分泌一氧化氮。另外，BCRC17010菌株（10⁸ cfu/mL）与HT - 29细胞共培养后，HT - 29细胞中的Bax/Bcl - 2比值为1.19，与其他对照组和乳酸菌组相比有显著差异（<0.05），因此推断BCRC17010菌株对HT - 29细胞具有促凋亡作用。BCRC14625菌株（10⁸ cfu/mL）与HT - 29细胞共培养4、8和12小时后，乳酸脱氢酶（LDH）活性显著增加（<0.05），对HT - 29细胞膜造成损伤；此外，与HT - 29细胞共培养8小时后，它诱导HT - 29细胞分泌一氧化氮（NO）。一些乳酸菌（LAB）菌株具有抑制结肠癌细胞系HT - 29 Bax/Bcl - 2信号通路或一氧化氮产生的能力。总之，我们证明了在体外测试的7株乳酸菌菌株中，BCRC17010菌株具有良好的黏附能力且LDH释放增加，是抑制HT - 29生长的最佳益生菌潜力菌株。
