Department of Biotechnology, Pondicherry University, Pondicherry, India.
Lett Appl Microbiol. 2011 Oct;53(4):481-7. doi: 10.1111/j.1472-765X.2011.03136.x. Epub 2011 Sep 6.
Lactobacillus plantarum AS1 was incubated with HT-29 adenocarcinoma cell line to assess its adhesion potency and examined for its inhibitory effect on the cell attachment by an enterovirulent bacterium Vibrio parahaemolyticus.
Lactobacillus plantarum AS1 attached efficiently to HT-29 cells as revealed by scanning electron microscopy and bacterial adhesion assay. Lactobacillus plantarum AS1 significantly reduced V. parahaemolyticus attached to HT-29 cells by competition, exclusion and displacement mode. Lactobacillus plantarum AS1 seems to adhere to human intestinal cells via mechanisms that involve different combinations of carbohydrate and protein factors on the bacteria and eukaryotic cell surface.
Strain Lact. plantarum AS1 inhibits the cell attachment of a pathogen V. parahaemolyticus by steric hindrance mechanism. Also, antibacterial factors such as bacteriocins, lactic acid and exopolysaccharides could be involved.
The ability to inhibit the adhesion of V. parahaemolyticus to intestinal cell line warrants further investigation to explore the use of probiotic strain Lact. plantarum AS1 in the management of gastroenteritis caused by V. parahaemolyticus.
将植物乳杆菌 AS1 与 HT-29 腺癌细胞系共培养，评估其黏附能力，并研究其对肠致病性副溶血性弧菌黏附细胞的抑制作用。
扫描电子显微镜和细菌黏附试验显示，植物乳杆菌 AS1 能有效地黏附 HT-29 细胞。植物乳杆菌 AS1 通过竞争、排除和置换模式显著减少了黏附于 HT-29 细胞的副溶血性弧菌。植物乳杆菌 AS1 似乎通过细菌和真核细胞表面上不同组合的碳水化合物和蛋白质因子来黏附于人类肠道细胞。
该研究表明，植物乳杆菌 AS1 株通过空间位阻机制抑制病原菌副溶血性弧菌的细胞黏附。此外，细菌素、乳酸和胞外多糖等抗菌因子也可能参与其中。
抑制副溶血性弧菌黏附于肠道细胞系的能力值得进一步研究，以探索使用益生菌株植物乳杆菌 AS1 来管理由副溶血性弧菌引起的胃肠炎。
