Department of Mechanical Engineering, Andong National University, Andong, Gyunbuk 760-749, South Korea.
J Biomater Sci Polym Ed. 2010;21(6-7):951-62. doi: 10.1163/156856209X458380.
In this study, we fabricated polycaprolactone/hydroxyapatite (PCL/HA) scaffolds with a multi-head deposition system, a solid free-form fabrication technology that was developed in our previous study. The bone regeneration potential of the scaffolds was compared with that of PCL scaffolds fabricated with the same system. The fabricated scaffolds had a pore size of 400 mum and a porosity of 66.7%. The PCL/HA scaffolds had higher mechanical strength and modulus than the PCL scaffolds. To compare the osteogenic potential, the two types of scaffolds were seeded with rat osteoblasts and cultured in vitro or implanted subcutaneously into athymic mice. The cells cultured on PCL/HA scaffolds expressed higher levels of osteopontin and osteonectin, both of which are osteogenic proteins. The PCL/HA scaffolds resulted in larger bone area and calcium deposition in the implants compared to the PCL scaffolds.
在这项研究中，我们使用多头沉积系统制造了聚己内酯/羟基磷灰石（PCL/HA）支架，这是我们之前的研究中开发的一种立体光固化成型技术。我们比较了支架的骨再生潜力与使用相同系统制造的 PCL 支架的骨再生潜力。所制造的支架的孔径为 400 微米，孔隙率为 66.7%。PCL/HA 支架的机械强度和模量均高于 PCL 支架。为了比较成骨潜力，将这两种类型的支架接种大鼠成骨细胞并在体外培养或皮下植入无胸腺小鼠。在 PCL/HA 支架上培养的细胞表达了更高水平的骨桥蛋白和骨粘连蛋白，这两种蛋白都是成骨蛋白。与 PCL 支架相比，PCL/HA 支架在植入物中产生了更大的骨面积和钙沉积。
