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超声冲洗对牙髓治疗中牙本质内消毒及氢氧化钙糊剂渗透的影响。

Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment.

作者信息

Arias Marcela Paola Castro, Maliza Amanda Garcia Alves, Midena Raquel Zanin, Graeff Márcia Sirlene Zardin, Duarte Marco Antonio Húngaro, Andrade Flaviana Bombarda de

机构信息

- Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Dentística, Endodontia e Materiais Odontológicos, Bauru, SP, Brasil.

- Universidade de São Paulo, Faculdade de Odontologia de Bauru, Centro Integrado de Pesquisa, Bauru, SP, Brasil.

出版信息

J Appl Oral Sci. 2016 Nov-Dec;24(6):575-581. doi: 10.1590/1678-775720150553.

DOI:10.1590/1678-775720150553
PMID:28076462
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5161256/
Abstract

OBJECTIVE

The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture.

MATERIAL AND METHODS

Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye.

RESULTS

The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05).

CONCLUSION

The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration with the fulfilment of the tubules.

摘要

目的

使用共聚焦激光扫描显微镜（CLSM）和微生物培养技术，评估氢氧化钙（CH）糊剂在感染牛牙本质中的抗菌效果及其渗透性。

材料与方法

采用新的污染方案，用粪肠球菌感染52颗牛牙；然后将其置入CH糊剂，并分为超声处理组和非超声处理组。使用普通尖端插入物进行1分钟的超声搅拌。15天后，CLSM通过死活细胞检测分析活菌和死菌。用牙钻收集牙本质壁碎屑，并计算菌落形成单位（CFU/mL）。使用B-罗丹明染料测试糊剂在牙本质小管内的渗透性。

结果

使用超声搅拌时，氢氧化钙糊剂显示出更好的效果（p<0.05）。

结论

CH糊剂的超声搅拌增强了其抗菌作用，并使其能够穿透牙本质小管并充满小管。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/84d911e2b7bb/1678-7757-jaos-24-6-0575-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/2c7167e8f326/1678-7757-jaos-24-6-0575-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/3b5027be7e13/1678-7757-jaos-24-6-0575-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/32f3a19be938/1678-7757-jaos-24-6-0575-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/1d7952a037cb/1678-7757-jaos-24-6-0575-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/84d911e2b7bb/1678-7757-jaos-24-6-0575-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/2c7167e8f326/1678-7757-jaos-24-6-0575-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/3b5027be7e13/1678-7757-jaos-24-6-0575-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/32f3a19be938/1678-7757-jaos-24-6-0575-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/1d7952a037cb/1678-7757-jaos-24-6-0575-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1dda/5161256/84d911e2b7bb/1678-7757-jaos-24-6-0575-gf05.jpg

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