Pereira Thais Cristina, Vasconcelos Layla Reginna Silva Munhoz De, Graeff Marcia Sirlene Zardin, Duarte Marco Antonio Hungaro, Bramante Clóvis Monteiro, Andrade Flaviana Bombarda De
a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
Acta Odontol Scand. 2017 Mar;75(2):87-93. doi: 10.1080/00016357.2016.1256427. Epub 2016 Nov 21.
The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.
Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules. In experiment of culture, the bacterial collection of the dentin fragments was done for counting the colony-forming units.
The tri-antibiotic paste had a slightly greater antimicrobial effect; however, there was no statistical difference between the groups.
It was concluded that the tri-antibiotic paste and the calcium hydroxide paste exercise the same effect on intra-tubular decontamination against E. faecalis. So, due the multiples advantages, the calcium hydroxide paste can be the choice for dentinal decontamination in regenerative procedures.
本研究旨在比较氢氧化钙糊剂和三联抗生素糊剂在体外牙本质内的抗菌能力。
将标准牛牙本质小管进行灭菌，然后通过一种新的牙本质深层污染方案用粪肠球菌进行感染。将标本用药物填充，分为两个试验组：氢氧化钙（第1组）和三联抗生素（第2组）糊剂。15天后，通过微生物培养和共聚焦激光扫描显微镜（CLSM）以及牙本质小管内活性染料检测LIVE/DEAD对牙齿进行评估。在培养实验中，对牙本质碎片的细菌收集物进行计数以计算菌落形成单位。
三联抗生素糊剂的抗菌效果略强；然而，两组之间无统计学差异。
得出的结论是，三联抗生素糊剂和氢氧化钙糊剂对牙本质小管内针对粪肠球菌的去污作用效果相同。因此，鉴于多种优势，氢氧化钙糊剂可作为再生治疗中牙本质去污的选择。
