Fimple Jacob Lee, Fontana Carla Raquel, Foschi Federico, Ruggiero Karriann, Song Xiaoqing, Pagonis Tom C, Tanner Anne C R, Kent Ralph, Doukas Apostolos G, Stashenko Philip P, Soukos Nikolaos S
Division of Endodontics, Harvard School of Dental Medicine, Boston, Massachusetts, USA.
J Endod. 2008 Jun;34(6):728-34. doi: 10.1016/j.joen.2008.03.011. Epub 2008 Apr 25.
We investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising Actinomyces israelii, Fusobacterium nucleatum subspecies nucleatum, Porphyromonas gingivalis, and Prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. The 4 test microorganisms were detected in root canals by using DNA probes. Scanning electron microscopy showed the presence of biofilms in root canals before therapy. Root canal systems were incubated with methylene blue (25 microg/mL) for 10 minutes followed by exposure to red light at 665 nm with an energy fluence of 30 J/cm(2). Light was delivered from a diode laser via a 250-microm diameter polymethyl methacrylate optical fiber that uniformly distributed light over 360 degrees. Photodynamic therapy (PDT) achieved up to 80% reduction of colony-forming unit counts. We concluded that PDT can be an effective adjunct to standard endodontic antimicrobial treatment when the PDT parameters are optimized.
我们在体外对拔除的人牙实验性感染根管内由以色列放线菌、具核梭杆菌具核亚种、牙龈卟啉单胞菌和中间普氏菌组成的多菌种根管生物膜研究了亚甲蓝的光动力效应。使用DNA探针在根管中检测到这4种测试微生物。扫描电子显微镜显示治疗前根管中存在生物膜。将根管系统用亚甲蓝（25微克/毫升）孵育10分钟，随后用能量通量为30焦/平方厘米的665纳米红光照射。光由二极管激光器通过直径250微米的聚甲基丙烯酸甲酯光纤传输，该光纤可在360度均匀分布光线。光动力疗法（PDT）使菌落形成单位计数减少高达80%。我们得出结论，当光动力疗法参数优化时，它可以作为标准牙髓抗菌治疗的有效辅助手段。
