Salgado Flávio L L, Artigiani-Neto Ricardo, Lopes-Filho Gaspar de Jesus
Postgraduate Program in Interdisciplinary Surgical Science, Paulista School of Medicine, Federal University of São Paulo - UNIFESP, São Paulo, SP, Brazil.
Arq Bras Cir Dig. 2016 Nov-Dec;29(4):223-226. doi: 10.1590/0102-6720201600040003.
Healing is an innate biological phenomenon, and carcinogenesis acquired, but with common humoral and cellular elements. Carcinogenesis interferes negatively in healing.
To evaluate the histological changes in laparotomy scars of healthy Balb/c mice and with an Ehrlich tumor in its various forms of presentation.
Fifty-four mice were divided into three groups of 18 animals. First group was the control; the second had Ehrlich tumor with ascites; and the third had the subcutaneous form of this tumor. Seven days after tumor inoculation, all 54 mice were submitted to laparotomy. All of the animals in the experiment were operated on again on 7th day after surgery, with resection of the scar and subsequent euthanasia of the animal. The scars were sent for histological assessment using immunohistochemical techniques to evaluate Cox-2 (cyclooxygenase 2), VEGF (vascular endothelial growth factor) and FGF (fibroblast growth factor). Semi-quantitatively analysis was done in the laparotomy scars and in the abdominal walls far away from the site of the operation.
Assessing the weight of the animals, the correct inoculation of the tumor and weight gain in the group with tumoral ascites was observed. The histological studies showed that groups with the tumor showed a statistically significant higher presence of Cox-2 compared to the control. In the Cox-2 analysis of the abdominal wall, the ascites group showed the most significant difference. VEGF did not present any significant differences between the three groups, regardless of the site. The FGF showed a significant increase in animals with the tumor.
Histological findings in both laparotomy scar and the abdominal wall showed that with Ehrlich's neoplasia there was an exacerbated inflammatory response, translated by more intense expression of Cox-2 and greater fibroblast proliferation, translated by more intense expression of FGF, that is, it stimulated both the immediate inflammatory reactions, observed with Cox-2 reactions, and late scarring by fibroblasts and FGF.
愈合是一种天生的生物学现象，而致癌作用是后天获得的，但二者具有共同的体液和细胞成分。致癌作用对愈合有负面影响。
评估健康的Balb/c小鼠以及患有不同表现形式艾氏腹水癌的小鼠剖腹手术瘢痕的组织学变化。
54只小鼠被分为三组，每组18只。第一组为对照组；第二组患有伴有腹水的艾氏肿瘤；第三组患有该肿瘤的皮下形式。肿瘤接种7天后，所有54只小鼠均接受剖腹手术。术后第7天，对实验中的所有动物再次进行手术，切除瘢痕并随后对动物实施安乐死。将瘢痕送去使用免疫组织化学技术进行组织学评估，以评估环氧化酶-2（Cox-2）、血管内皮生长因子（VEGF）和成纤维细胞生长因子（FGF）。对剖腹手术瘢痕以及远离手术部位的腹壁进行半定量分析。
通过评估动物体重，观察到肿瘤腹水组的肿瘤接种正确且体重增加。组织学研究表明，与对照组相比，肿瘤组中Cox-2的存在在统计学上显著更高。在腹壁的Cox-2分析中，腹水组差异最为显著。三组之间的VEGF在任何部位均未呈现任何显著差异。FGF在患有肿瘤的动物中显著增加。
剖腹手术瘢痕和腹壁的组织学结果均表明，患有艾氏肿瘤时存在炎症反应加剧的情况，表现为Cox-2表达更强烈，成纤维细胞增殖更明显，表现为FGF表达更强烈，也就是说，它既刺激了由Cox-2反应观察到的即时炎症反应，也刺激了成纤维细胞和FGF引起的后期瘢痕形成。
