Atasoy Kerem Turgut, Korkmaz Yavuz Tolga, Odaci Ersan, Hanci Hatice
Karadeniz Technical University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Trabzon, Turkey.
Karadeniz Technical University, Faculty of Medicine, Department of Histology and Embriology, Trabzon, Turkey.
Braz Oral Res. 2017 Jan 5;31:e7. doi: 10.1590/1807-3107BOR-2017.vol31.0007.
The aim of this study was to evaluate the efficacy of low-level 940 nm laser therapy with energy intensities of 5, 10 and 20 J/cm2 on bone healing in an animal model. A total of 48 female adult Wistar rats underwent surgery to create bone defects in the right tibias. Low-level laser therapy (LLLT) was applied immediately after surgery and on post-operative days 2, 4, 6, 8, 10 and 12 in three study groups with energy intensities of 5 J/cm2, 10 J/cm2 and 20 J/cm2 using a 940 nm Gallium-Aluminium-Arsenide (Ga-Al-As) laser, while one control group underwent only the tibia defect surgery. All animals were sacrificed 4 or 8 weeks post-surgery. Fibroblasts, osteoblasts, osteocytes, osteoclasts and newly formed vessels were evaluated by a histological examination. No significant change was observed in the number of osteocytes, osteoblasts, osteoclasts and newly formed vessels at either time period across all laser groups. Although LLLT with the 10 J/cm2 energy density increased fibroblast activity at the 4th week in comparison with the 5 and 20 J/cm2 groups, no significant change was observed between the laser groups and the control group. These results indicate that low-level 940 nm laser with different energy intensities may not have marked effects on the bone healing process in both phases of bone formation.
本研究旨在评估能量强度为5、10和20 J/cm²的940 nm低强度激光疗法对动物模型骨愈合的疗效。总共48只成年雌性Wistar大鼠接受手术，在右胫骨制造骨缺损。三个研究组在术后即刻以及术后第2、4、6、8、10和12天，使用940 nm砷化镓铝（Ga-Al-As）激光分别给予能量强度为5 J/cm²、10 J/cm²和20 J/cm²的低强度激光疗法（LLLT），而一个对照组仅接受胫骨缺损手术。所有动物在术后4周或8周处死。通过组织学检查评估成纤维细胞、成骨细胞、骨细胞、破骨细胞和新形成的血管。在所有激光组的两个时间段内，骨细胞、成骨细胞、破骨细胞和新形成血管的数量均未观察到显著变化。尽管与5 J/cm²和20 J/cm²组相比，能量密度为10 J/cm²的LLLT在第4周时增加了成纤维细胞活性，但激光组与对照组之间未观察到显著变化。这些结果表明，不同能量强度的940 nm低强度激光可能对骨形成两个阶段的骨愈合过程均无显著影响。
