低强度激光疗法联合富含白细胞和血小板的纤维蛋白对兔颅骨缺损骨再生的影响：组织学和组织形态计量学研究
The Effect of Low-Level Laser Therapy in Combination with Leukocyte- and Platelet- Rich Fibrin on Bone Regeneration in Rabbits' Calvarial Defects: Histologic and Histomorphometric Studies.
作者信息
Shanei Fereshteh, Khoshzaban Ahad, Taleghani Ferial, Tehranchi Maryam, Tayeed Mohammad Hossein
机构信息
Department of Periodontics, Faculty of Dentistry, Alborz University of Medical Sciences, Alborz, Iran.
Iranian Tissue Bank Research Center, Imam Khomeini Medical Complex, Tehran University of Medical Sciences, Tahran, Iran.
出版信息
Cell J. 2022 Jun;24(6):346-352. doi: 10.22074/cellj.2022.7864. Epub 2022 Jun 29.
OBJECTIVE
Bone regeneration is a desired treatment outcome in implant dentistry. The primary goal of the current investigation was to assess the joint effect of low-level laser therapy (LLLT) and leukocyte- and platelet-rich fibrin (PRF) on new bone formation.
MATERIALS AND METHODS
During this experiment study, forty bone defects (8 mm in diameter) were generated in the calvaria of ten New-Zealand white rabbits. defects were filled with autogenous bone defined as the control group, autogenous bone with leukocyte- and PRF (PRF group), autogenous bone and low-level diode laser radiation (LLLT group), and autogenous bone with leukocyte- and PRF and low-level laser radiation (LP group). Laser irradiation was done every second day for 2 weeks after surgery. Five rabbits were randomly selected to be sacrificed on postoperative weeks 4 and 8. On one and two-month post-surgery, histological and histomorphometric parameters including bone formation, fibroblast, and osteoblast were assessed.
RESULTS
The histological panel depicted that the ratio of fresh bone formation increased at one-and two-month postsurgery in all treatment groups compared to the control group. The most favorable results were seen in the LP group, followed by the PRF group. Based on the ANOVA test, bone neoformation was statistically significant in the LP group in comparison with the control group (P<0.001). One-month post-surgery, a higher degree of fibroblast was seen in the control group, while the last place was for LP group (118.6 ± 6.9 vs. 24.0 ± 3.2). In the PRF group, the percentage of bone formation was higher than that in the control group (13.2 ± 2.8 vs. 2.0 ± 1.2), but no significant difference when compared to the LP group (13.2 ± 2.8 vs. 19.0 ±.3.8).
CONCLUSION
The combined L-PRF and LLLT was more likely to have a positive effect on accelerating bone regeneration and reducing fibrosis.
目的
骨再生是种植牙治疗中期望达到的结果。本研究的主要目的是评估低强度激光疗法（LLLT）和富白细胞血小板纤维蛋白（PRF）对新骨形成的联合作用。
材料与方法
在本实验研究中，在10只新西兰白兔的颅骨上制造了40个骨缺损（直径8毫米）。缺损分别用自体骨填充作为对照组、自体骨加富白细胞血小板纤维蛋白（PRF组）、自体骨加低强度二极管激光照射（LLLT组）以及自体骨加富白细胞血小板纤维蛋白和低强度激光照射（LP组）。术后每隔一天进行激光照射，持续2周。随机选择5只兔子在术后第4周和第8周处死。在术后1个月和2个月时，评估包括骨形成、成纤维细胞和成骨细胞在内的组织学和组织形态计量学参数。
结果
组织学结果显示，与对照组相比，所有治疗组在术后1个月和2个月时新鲜骨形成的比例均增加。LP组的效果最理想，其次是PRF组。基于方差分析测试，LP组的骨新生与对照组相比具有统计学意义（P<0.001）。术后1个月，对照组的成纤维细胞程度较高，而LP组最低（118.6±6.9对24.0±3.2）。在PRF组中，骨形成百分比高于对照组（13.2±2.8对2.0±1.2），但与LP组相比无显著差异（13.2±2.8对19.0±3.8）。
结论
联合应用PRF和LLLT更有可能对加速骨再生和减少纤维化产生积极作用。
Zotero 插件