Shintani Masaki
a Department of Engineering , Graduate School of Integrated Science and Technology, Shizuoka University , Hamamatsu , Japan.
b Department of Bioscience , Graduate School of Science and Technology, Shizuoka University , Hamamatsu , Japan.
Biosci Biotechnol Biochem. 2017 May;81(5):854-862. doi: 10.1080/09168451.2016.1270743. Epub 2017 Jan 12.
Mobile genetic elements (MGEs) including plasmids have an important role in the rapid evolution and adaptation of bacteria. Here, the behavior of MGEs in different environments is reviewed, in particular, behavior of the plasmid pCAR1, a carbazole-degradative plasmid isolated from Pseudomonas resinovorans CA10. pCAR1 belongs to incompatibility P-7 group and is self-transmissible among different bacteria. Comparisons of changes in the transcriptome of different host strains caused by carrying pCAR1 revealed common responses in the hosts and host-specific responses. Monitoring the survival of the host and transfer of the plasmid in artificial and natural environmental samples revealed several environmental factors, including cations and water content, which changed the behavior of both the host and its plasmid. Single-cell level analysis to detect the transconjugants of different plasmids successfully determined the transfer range of the plasmids. Three nucleoid-associated proteins encoded on pCAR1 are important factors affecting its genetic stability, maintenance, and transfer.
包括质粒在内的移动遗传元件（MGEs）在细菌的快速进化和适应过程中发挥着重要作用。在此，我们综述了MGEs在不同环境中的行为，特别是质粒pCAR1的行为，pCAR1是一种从树脂糖假单胞菌CA10中分离出的咔唑降解质粒。pCAR1属于不相容性P-7组，可在不同细菌之间自我传递。对携带pCAR1的不同宿主菌株转录组变化的比较揭示了宿主中的共同反应以及宿主特异性反应。监测宿主在人工和自然环境样品中的存活情况以及质粒的转移情况，发现了几个环境因素，包括阳离子和含水量，它们改变了宿主及其质粒的行为。通过单细胞水平分析检测不同质粒的接合子成功确定了质粒的转移范围。pCAR1上编码的三种类核相关蛋白是影响其遗传稳定性、维持和转移的重要因素。
