Sawan Saly, Yaacoub Tania, Hraoui-Bloquet Souad, Sadek Riyad, Hleihel Walid, Fajloun Ziad, Karam Marc
Department of Biology, University of Balamand, Kourah, Lebanon.
Faculty of Sciences II (Fanar), Lebanese University, B.P. 90656 Jdeidet El Maten, Lebanon.
Exp Toxicol Pathol. 2017 Apr 4;69(4):173-178. doi: 10.1016/j.etp.2017.01.001. Epub 2017 Jan 8.
The Viperidae family venom is a rich source of bioactive compounds such as many proteases, which cause tissue necrosis and affect mostly the vascular system. However, the venom exhibits therapeutic potentials and has contributed to the development of some medical drugs. Specifically, the Montivipera bornmuelleri venom has shown to exhibit antibacterial, pro-inflammatory and antifungal activities.
This work evaluates the cytotoxic effect of the M. bornmuelleri venom on human-derived keratinocytes including the non-tumorigenic HaCaT, the benign A5 and the low-grade malignant II4 cells.
The toxicity of different venom concentrations (0.9, 1.87, 3.75, 7.5, 15, 30 and 60μg/mL) and their effect on the viability of the cells lines were assessed using the Lactate Dehydrogenase (LDH) activity and the Trypan blue tests after 24h of incubation.
The venom was able to reduce the viability of all cell lines in a dose dependent manner with the HaCat cells being the least affected. For example, the 60μg/mL dose induced a more significant decrease the viability of A5 (44%) and II4 (21.33%) keratinocytes as compared to HaCaT cells (70.63%). Also, this venom showed a higher cytotoxic activity on the A5 (52.45%) and II4 (98.67%) cells as compared to HaCaT cells (30.14%) with an IC estimated at 10μg/mL on II4 and at 60μg/mL on benign A5.
Those differential cytotoxic effects of the M. bornmuelleri venom pave the road for more advanced studies which might unravel the potential anticancer effects of this venom.
蝰蛇科蛇毒是多种生物活性化合物的丰富来源，例如许多蛋白酶，这些蛋白酶会导致组织坏死，并且主要影响血管系统。然而，这种毒液具有治疗潜力，并且对一些药物的开发有贡献。具体而言，波氏山蝰毒液已显示出抗菌、促炎和抗真菌活性。
本研究评估波氏山蝰毒液对人源角质形成细胞的细胞毒性作用，包括非致瘤性的HaCaT细胞、良性的A5细胞和低级别恶性的II4细胞。
在孵育24小时后，使用乳酸脱氢酶（LDH）活性和台盼蓝试验评估不同毒液浓度（0.9、1.87、3.75、7.5、15、30和60μg/mL）的毒性及其对细胞系活力的影响。
毒液能够以剂量依赖性方式降低所有细胞系的活力，其中HaCaT细胞受影响最小。例如，与HaCaT细胞（70.63%）相比，60μg/mL剂量导致A5（44%）和II4（21.33%）角质形成细胞的活力更显著下降。此外，与HaCaT细胞（30.14%）相比，这种毒液对A5（52.45%）和II4（98.67%）细胞显示出更高的细胞毒性活性，估计对II4细胞的IC为10μg/mL，对良性A5细胞的IC为60μg/mL。
波氏山蝰毒液的这些不同细胞毒性作用为更深入的研究铺平了道路，这些研究可能揭示这种毒液潜在的抗癌作用。
