Faberowski N, Stefansson E, Davidson R C
Duke University Medical Center, Department of Ophthalmology, Durham, North Carolina.
Invest Ophthalmol Vis Sci. 1989 Nov;30(11):2309-13.
We developed a quantitative histologic method for assessing injury in the rat retina due to transient ischemia. We used this technique to test the effectiveness of local hypothermia and allopurinol, an inhibitor of oxygen-free radical formation, in reducing ischemia/reperfusion injury in the rat retina. Retinal ischemia and reperfusion was produced by transient ligation of the optic nerve. Histologic evaluation by a masked observer was based on the average count of nonpyknotic nuclei in the inner nuclear layer of the retina from eight high power fields (X100) in one 5 microns thick sagital section at or near the optic nerve. A sharp increase in tissue damage occurs between 90 and 120 min of ischemia. Ischemia for periods of 60 and 90 min produced mild damage while periods of 120 and 240 min produced severe damage. Hypothermia protected the retina significantly from 120 min of ischemic injury (P less than 0.001 student t-test, compared to 120 min control), while allopurinol had no protective effect.
我们开发了一种定量组织学方法，用于评估大鼠视网膜因短暂缺血所致的损伤。我们运用该技术来测试局部低温以及别嘌呤醇（一种氧自由基形成抑制剂）在减轻大鼠视网膜缺血/再灌注损伤方面的有效性。通过短暂结扎视神经来造成视网膜缺血和再灌注。由一位不知情的观察者进行组织学评估，其依据是在视神经处或其附近一个5微米厚的矢状切片中，来自八个高倍视野（×100）的视网膜内核层中非固缩核的平均计数。在缺血90至120分钟之间，组织损伤会急剧增加。缺血60分钟和90分钟会造成轻度损伤，而缺血120分钟和240分钟则会造成严重损伤。低温显著保护视网膜免受120分钟的缺血损伤（与120分钟的对照组相比，学生t检验，P小于0.001），而别嘌呤醇则没有保护作用。
