Wang Hui, Hu Wei, Liang Zhiling, Zeng Lu, Li Jianjie, Yan Hao, Yang Pingchang, Liu Zhigang, Wang Lianglu
Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University3688 Nanhai Blvd, Shenzhen 518060, China.
Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University Shenzhen 518060, China.
Am J Transl Res. 2016 Dec 15;8(12):5320-5329. eCollection 2016.
(, also known as silkworm) plays a role in the pathogenesis of allergic diseases. However, its allergens are to be characterized. The aim of this paper is to identify new silkworm allergens. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify potential allergens from silkworm pupa. Six potential allergens were identified in this study. Thiol peroxiredoxin (TP), one of the 6 allergens, reacted to serum IgE from patients sensitized to silkworm. By sensitizing with TP allergic asthma like symptoms were induced in mice, including elevation of the levels of serum IgE, IL-4 from bronchoalveolar lavage fluid and culture supernatant of spleen cells. In vitro experiments showed that TP significantly induced RAW264.7 cells (a macrophage cell line) apoptosis via modulating the BCL2 and Caspase9 pathways. The levels of CD80, CD40, CD83 and TNF-α in DC2.4 cells (a dendritic cell line) were increased in the culture after exposure to TP. In summary, TP is an allergic component of silkworm. It induces allergic asthma, and modulates the functions of macrophages and dendritic cells.
（，也被称为家蚕）在过敏性疾病的发病机制中起作用。然而，其过敏原有待鉴定。本文旨在鉴定新的家蚕过敏原。采用二维凝胶电泳（2-DE）和质谱法从家蚕蛹中分离和鉴定潜在过敏原。本研究鉴定出6种潜在过敏原。硫醇过氧化物酶（TP）是6种过敏原之一，与对家蚕致敏患者的血清IgE发生反应。通过用TP致敏，在小鼠中诱导出类似过敏性哮喘的症状，包括血清IgE、支气管肺泡灌洗液和脾细胞培养上清液中IL-4水平升高。体外实验表明，TP通过调节BCL2和Caspase9途径显著诱导RAW264.7细胞（一种巨噬细胞系）凋亡。在暴露于TP后的培养中，DC2.4细胞（一种树突状细胞系）中CD80、CD40、CD83和TNF-α的水平升高。总之，TP是家蚕的一种过敏成分。它诱导过敏性哮喘，并调节巨噬细胞和树突状细胞的功能。
