Yi Haijin, Guo Weiwei, Chen Wei, Chen Lei, Ye Jingying, Yang Shiming
Department of Otolaryngology, Head & Neck Surgery, Beijing Tsinghua Changgung Hospital, Medical Center, Tsinghua University168 Litang Street, Changping District, Beijing, China; Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology of PLA, Chinese PLA General HospitalBeijing 100853, China.
Department of Otolaryngology, Head & Neck Surgery, Institute of Otolaryngology of PLA, Chinese PLA General Hospital Beijing 100853, China.
Am J Transl Res. 2016 Dec 15;8(12):5494-5502. eCollection 2016.
The objective of this paper was to investigate the suitability of the miniature pig as a large animal model of cochlear implantation (CI). Micro-CT scanning and three-dimensional reconstructions of the inner ear were completed in six animals. Photographs of the procedures and measurements of the inner ear were made. The CI procedure was simulated in 10 animals. Electrically evoked auditory brain stem responses (EABRs) and radiographic images were evaluated during or after the CI procedure. Morphological examination and measurements of inner ears of the miniature pigs were completed by micro-CT scanning. The height of the scala tympani was 873.12 µm in the 1st turn, 641.46 µm in the 2nd turn, 445.13 µm in the third turn and 339.19 µm in the fourth turn. The length of the cochlea was 38.6 mm, larger than other animal models (7.2 mm in rats and 22 mm in macaque, for example) and similar to that in the human (36 mm). Commercial electrodes used in humans (870 µm at the end and 630 µm at the tip in diameter) were implanted in the pig's cochlea, through which normal eABRs were obtained. Radiographic images after the CI procedure revealed electrodes located in the scala tympani of the first and second turns. Compared with traditional animal models, greater similarities of the inner ear between miniature pigs and humans make this animal a potentially useful model for CI studies.
本文的目的是研究小型猪作为人工耳蜗植入（CI）大型动物模型的适用性。对6只动物进行了内耳的微型计算机断层扫描（Micro-CT）和三维重建。拍摄了手术过程照片并对内耳进行了测量。在10只动物身上模拟了CI手术。在CI手术期间或之后评估了电诱发听性脑干反应（EABR）和影像学图像。通过微型计算机断层扫描完成了小型猪内耳的形态学检查和测量。鼓阶高度在第一圈为873.12 µm，第二圈为641.46 µm，第三圈为445.13 µm，第四圈为339.19 µm。耳蜗长度为38.6 mm，大于其他动物模型（例如大鼠为7.2 mm，猕猴为22 mm），与人类的耳蜗长度（36 mm）相似。将人类使用的商用电极（末端直径为870 µm，尖端直径为630 µm）植入猪的耳蜗，通过该电极获得了正常的EABR。CI手术后的影像学图像显示电极位于第一圈和第二圈的鼓阶。与传统动物模型相比，小型猪和人类内耳的相似性更高，这使得这种动物成为CI研究的潜在有用模型。
