Zhao Ning, Liu Yanjuan, Liang Hongfeng, Jiang Xuejun
Department of E. N. T., The First Hospital Affiliated of China Medical University 155 Nanjing Bei Street, Shenyang 110001, China.
Department of E. N. T., The First Hospital Affiliated of Dalian Medical University 222 Zhongshan Road, Dalian 116013, China.
Am J Transl Res. 2016 Dec 15;8(12):5628-5636. eCollection 2016.
To determine the potential of bone marrow-derived mesenchymal stem cells (BMSCs) for immunomodulatory mechanism in mice model of allergic rhinitis (AR).
BMSCs were isolated and the surface markers and stemness were analyzed. The effect of BMSCs was evaluated in BALB/c mice that were randomly divided into three groups (control group, ovalbumin (OVA) group, OVA+BMSCs group). BMSCs were administered intravenously to OVA sensitized mice on days 1, 7, 14 and 21, and subsequent OVA challenge was conducted daily from days 22 to 35. Several parameters of allergic inflammation were assessed.
Mesenchymal stem cells can be successfully isolated from bone marrow of mice. Intravenous injection of BMSCs significantly reduced allergic symptoms, eosinophil infiltration, OVA-specific immunoglobulin E (IgE), T-helper 2 (Th2) cytokine profile (interleukin (IL)-4, IL-5 and IL-13) and regulatory cytokines (IL-10). In addition, level of Th1 (IFN-γ) was significantly increased.
Administration of BMSCs effectively reduced allergic symptoms and inflammatory parameters in the mice model of AR. BMSCs treatment is potentially an alternative therapeutic modality in AR.
在变应性鼻炎（AR）小鼠模型中确定骨髓间充质干细胞（BMSCs）的免疫调节机制潜力。
分离BMSCs并分析其表面标志物和干性。在随机分为三组（对照组、卵清蛋白（OVA）组、OVA + BMSCs组）的BALB/c小鼠中评估BMSCs的作用。在第1、7、14和21天对OVA致敏小鼠静脉注射BMSCs，从第22天至35天每天进行后续OVA激发。评估变应性炎症的几个参数。
可从小鼠骨髓中成功分离间充质干细胞。静脉注射BMSCs显著减轻变应性症状、嗜酸性粒细胞浸润、OVA特异性免疫球蛋白E（IgE）、辅助性T细胞2（Th2）细胞因子谱（白细胞介素（IL）-4、IL-5和IL-13）以及调节性细胞因子（IL-10）。此外，Th1（干扰素-γ）水平显著升高。
给予BMSCs可有效减轻AR小鼠模型中的变应性症状和炎症参数。BMSCs治疗可能是AR的一种替代治疗方式。
