Shi Zhaohui, Jiang Weihong, Chen Xiaodong, Xu Min, Wang Jian, Lai Yubin, Zha Dingjun
Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Air Force Millitary Medical University, Xi'an, Shaanxi Province, China.
Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Biosci Rep. 2020 Nov 27;40(11). doi: 10.1042/BSR20201643.
Allergic rhinitis (AR) is a non-infectious chronic inflammatory disease of nasal mucosa provoking T helper cell (Th) 17 response. Chlorogenic acid (CGA), one of the most abundant polyphenol compounds in various agricultural products, possesses antiviral, anti-inflammatory, and antibacterial properties. However, the effect of CGA on AR is unclear. Thus, our study explored the effect of CGA in modulating AR-related symptoms and immunoreaction, especially Th17 response. AR mice were induced by ovalbumin (OVA) administration and further treated with CGA or dexamethasone (Dex). The frequencies of rubbing and sneezing of AR mice were recorded. Histopathological analysis of nasal mucosa was conducted by Hematoxylin-Eosin and Periodic acid-Schiff stainings. The serum and nasal mucosa levels of OVA-immunoglobulin (Ig)E, interferon (IFN)-γ, retinoic acid-associated nuclear orphan receptor (ROR)-γt, and interleukin (IL)-17A were measured by enzyme-linked immunosorbent assay, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), or Western blot. The ratio of CD4+IL-17+Th17 cells to CD4+ T cells in peripheral blood of AR mice was assessed by flow cytometer. CGA diminished the frequencies of rubbing and sneezing of AR mice in a concentration-dependent manner. CGA attenuated histopathological abnormalities and decreased goblet cell number in nasal mucosa of AR mice. CGA decreased the serum levels of OVA-IgE, ROR-γt, and IL-17A, while increasing the serum level of IFN-γ in AR mice. Meanwhile, CGA decreased the ratio of CD4+IL-17+Th17 cells to CD4+T cells in peripheral blood and the mRNA and protein levels of IL-17A and ROR-γt in AR mice. CGA ameliorated AR-related symptoms in mice by regulating Th17 cells, which could be a candidate for the treatment of AR.
变应性鼻炎（AR）是一种引发T辅助细胞（Th）17反应的鼻黏膜非感染性慢性炎症性疾病。绿原酸（CGA）是各种农产品中含量最为丰富的多酚化合物之一，具有抗病毒、抗炎和抗菌特性。然而，CGA对AR的作用尚不清楚。因此，我们的研究探讨了CGA在调节AR相关症状和免疫反应，尤其是Th17反应方面的作用。通过给予卵清蛋白（OVA）诱导建立AR小鼠模型，并进一步用CGA或地塞米松（Dex）进行治疗。记录AR小鼠的蹭鼻和打喷嚏频率。采用苏木精-伊红染色和高碘酸-希夫染色对鼻黏膜进行组织病理学分析。通过酶联免疫吸附测定、定量逆转录聚合酶链反应（qRT-PCR）或蛋白质免疫印迹法检测OVA免疫球蛋白（Ig）E、干扰素（IFN）-γ、维甲酸相关核孤儿受体（ROR）-γt和白细胞介素（IL）-17A的血清和鼻黏膜水平。通过流式细胞仪评估AR小鼠外周血中CD4 + IL-17 + Th17细胞与CD4 + T细胞的比例。CGA以浓度依赖性方式降低AR小鼠的蹭鼻和打喷嚏频率。CGA减轻了AR小鼠鼻黏膜的组织病理学异常并减少了杯状细胞数量。CGA降低了AR小鼠血清中OVA-IgE、ROR-γt和IL-17A的水平，同时提高了血清中IFN-γ的水平。与此同时，CGA降低了AR小鼠外周血中CD4 + IL-17 + Th17细胞与CD4 + T细胞的比例以及IL-17A和ROR-γt的mRNA和蛋白质水平。CGA通过调节Th17细胞改善了小鼠的AR相关症状，这可能是一种治疗AR的候选药物。
