Farshid Amir Abbas, Tamaddonfard Esmaeal, Moradi-Arzeloo Masoumeh, Mirzakhani Navideh
Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Avicenna J Phytomed. 2016 Nov-Dec;6(6):658-670.
Crocin is a saffron constituent with a potent anti-oxidant activity. The present study investigated the effects of crocin and insulin treatments (alone or in combination) on cardiac function and pathology in diabetic rats.
Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (STZ, 50 mg/kg). Thereafter, crocin (5, 10 and 20 mg/kg, i.p.), subcutaneous (s.c.) injection of insulin (4 IU/kg) and their combination were administered for eight weeks. Blood glucose level and whole heart and body weights were measured. Electrocardiography (ECG) was carried out using the lead II. Serum concentrations of lactate dehydrogenase (LDH), creatine kinase-MB isoenzyme (CK-MB), and the heart tissue malodialdehyde (MDA) and superoxide dismutase (SOD) contents were determined. The heart lesions were evaluated by light microscopy.
STZ decreased body weight and increased whole heart weight/body weight ratio. It also decreased heart rate, and increased RR and QT intervals and T wave amplitude. STZ increased blood glucose, serum LDH and CK-MB levels, augmented heart tissue MDA content, decreased SOD content of heart tissue, and produced hemorrhages, degeneration, interstitial edema, and fibroblastic proliferation in the heart tissue. Crocin (10 and 20 mg/kg, i.p.), insulin (4 IU/kg, s.c.) and their combination (5 mg/kg of crocin with 4 IU/kg of insulin) treatments recovered the ECG, biochemical and histopathological changes induced by STZ.
The results showed cardioprotective effects of crocin and insulin in STZ-induced diabetic rats. The antioxidant and anti-hyperglycemic properties of crocin and insulin may be involved in their cardioprotective actions.
西红花苷是藏红花的一种成分，具有强大的抗氧化活性。本研究调查了西红花苷和胰岛素单独或联合治疗对糖尿病大鼠心脏功能和病理的影响。
通过腹腔注射链脲佐菌素（STZ，50 mg/kg）诱导糖尿病。此后，腹腔注射西红花苷（5、10和20 mg/kg）、皮下注射胰岛素（4 IU/kg）及其联合用药，持续八周。测量血糖水平、全心重量和体重。使用II导联进行心电图检查。测定血清乳酸脱氢酶（LDH）、肌酸激酶-MB同工酶（CK-MB）浓度以及心脏组织丙二醛（MDA）和超氧化物歧化酶（SOD）含量。通过光学显微镜评估心脏病变。
STZ降低了体重，增加了全心重量/体重比。它还降低了心率，增加了RR和QT间期以及T波振幅。STZ增加了血糖、血清LDH和CK-MB水平，增加了心脏组织MDA含量，降低了心脏组织SOD含量，并在心脏组织中产生了出血、变性、间质水肿和成纤维细胞增殖。西红花苷（腹腔注射10和20 mg/kg）、胰岛素（皮下注射4 IU/kg）及其联合用药（5 mg/kg西红花苷与4 IU/kg胰岛素）治疗恢复了由STZ诱导的心电图、生化和组织病理学变化。
结果显示西红花苷和胰岛素对STZ诱导的糖尿病大鼠具有心脏保护作用。西红花苷和胰岛素的抗氧化和降血糖特性可能与其心脏保护作用有关。
