• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

耐甲氧西林生物膜及其对细菌黏附和聚集的影响。

Methicillin-Resistant Biofilms and Their Influence on Bacterial Adhesion and Cohesion.

作者信息

Dakheel Khulood Hamid, Abdul Rahim Raha, Neela Vasantha Kumari, Al-Obaidi Jameel R, Hun Tan Geok, Yusoff Khatijah

机构信息

Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.

出版信息

Biomed Res Int. 2016;2016:4708425. doi: 10.1155/2016/4708425. Epub 2016 Dec 18.

DOI:10.1155/2016/4708425
PMID:28078291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5203895/
Abstract

Twenty-five methicillin-resistant (MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype was type t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested.

摘要

通过葡萄球菌蛋白A基因分型和形成生物膜的能力对25株耐甲氧西林金黄色葡萄球菌（MRSA）分离株进行了鉴定。采用分散试验评估生物膜中胞外多糖、蛋白质以及细胞外DNA和RNA的存在情况。此外，分别使用填充珠法和机械破坏法评估细胞对表面的粘附和细胞凝聚情况。主要基因型为t127型（25株分离株中有22株）；大多数分离株被归类为中度生物膜产生菌。12株分离株表现出不依赖PIA的生物膜形成，而其余13株分离株则依赖PIA。两组在经核糖核酸酶和脱氧核糖核酸酶消化后再用蛋白酶K处理后均表现出强烈的分散。依赖PIA的生物膜在高碘酸钠处理后表现出不同程度的分散，而不依赖PIA的生物膜则表现出生物膜形成增强。生物膜形成程度或生物膜成分与细菌的粘附或凝聚能力之间没有相关性，但生物膜耗尽后对玻璃珠的粘附效率增加。总之，核酸和蛋白质构成了MRSA克隆t127生物膜基质的主要成分，并且在所测试的生物膜中粘附和凝聚之间似乎存在关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/3eae40da2bed/BMRI2016-4708425.013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/214daff51c43/BMRI2016-4708425.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a657ce6c211c/BMRI2016-4708425.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/b60e72de417c/BMRI2016-4708425.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a09823afc9d2/BMRI2016-4708425.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/5270f47c3ac0/BMRI2016-4708425.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/89c786f222ea/BMRI2016-4708425.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/df804bee7502/BMRI2016-4708425.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/4523bd515137/BMRI2016-4708425.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/0cc9d71013f1/BMRI2016-4708425.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/2101798b2851/BMRI2016-4708425.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/3ba0b09d5d36/BMRI2016-4708425.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a8ea95840126/BMRI2016-4708425.012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/3eae40da2bed/BMRI2016-4708425.013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/214daff51c43/BMRI2016-4708425.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a657ce6c211c/BMRI2016-4708425.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/b60e72de417c/BMRI2016-4708425.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a09823afc9d2/BMRI2016-4708425.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/5270f47c3ac0/BMRI2016-4708425.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/89c786f222ea/BMRI2016-4708425.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/df804bee7502/BMRI2016-4708425.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/4523bd515137/BMRI2016-4708425.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/0cc9d71013f1/BMRI2016-4708425.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/2101798b2851/BMRI2016-4708425.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/3ba0b09d5d36/BMRI2016-4708425.011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/a8ea95840126/BMRI2016-4708425.012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a342/5203895/3eae40da2bed/BMRI2016-4708425.013.jpg

相似文献

1
Methicillin-Resistant Biofilms and Their Influence on Bacterial Adhesion and Cohesion.耐甲氧西林生物膜及其对细菌黏附和聚集的影响。
Biomed Res Int. 2016;2016:4708425. doi: 10.1155/2016/4708425. Epub 2016 Dec 18.
2
Biofilm formation and dispersal of Staphylococcus aureus under the influence of oxacillin.金黄色葡萄球菌在苯唑西林影响下的生物膜形成和分散。
Microb Pathog. 2013 Aug-Sep;61-62:66-72. doi: 10.1016/j.micpath.2013.05.002. Epub 2013 May 25.
3
Correlation Between Biofilm Formation and Antibiotic Resistance in MRSA and MSSA Isolated from Clinical Samples in Iran: A Systematic Review and Meta-Analysis.伊朗临床样本中分离的 MRSA 和 MSSA 中生物膜形成与抗生素耐药性的相关性：系统评价和荟萃分析。
Microb Drug Resist. 2020 Sep;26(9):1071-1080. doi: 10.1089/mdr.2020.0001. Epub 2020 Mar 10.
4
Human methicillin-sensitive Staphylococcus aureus biofilms: potential associations with antibiotic resistance persistence and surface polysaccharide antigens.人源甲氧西林敏感金黄色葡萄球菌生物膜：与抗生素耐药性持久性及表面多糖抗原的潜在关联
J Basic Microbiol. 2014 Jul;54(7):721-8. doi: 10.1002/jobm.201200557. Epub 2013 May 17.
5
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) isolates of swine origin form robust biofilms.猪源耐甲氧西林金黄色葡萄球菌（LA-MRSA）分离株形成坚固的生物膜。
PLoS One. 2013 Aug 9;8(8):e73376. doi: 10.1371/journal.pone.0073376. eCollection 2013.
6
Comparative characterisation of genotypically different clones of MRSA in the production of biofilms.耐甲氧西林金黄色葡萄球菌（MRSA）基因分型不同的克隆株在生物膜形成中的比较特性分析
J Biomed Biotechnol. 2012;2012:417247. doi: 10.1155/2012/417247. Epub 2012 Mar 14.
7
Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage.侵袭性金黄色葡萄球菌分离株中的生物膜形成与克隆谱系有关。
Microb Pathog. 2016 Jan;90:41-9. doi: 10.1016/j.micpath.2015.10.023. Epub 2015 Nov 4.
8
Sustained Nitric Oxide-Releasing Nanoparticles Interfere with Methicillin-Resistant Staphylococcus aureus Adhesion and Biofilm Formation in a Rat Central Venous Catheter Model.持续释放一氧化氮的纳米颗粒在大鼠中心静脉导管模型中干扰耐甲氧西林金黄色葡萄球菌的黏附和生物膜形成。
Antimicrob Agents Chemother. 2016 Dec 27;61(1). doi: 10.1128/AAC.02020-16. Print 2017 Jan.
9
Adhesion and biofilm formation by Staphylococcus aureus clinical isolates under conditions relevant to the host: relationship with macrolide resistance and clonal lineages.金黄色葡萄球菌临床分离株在与宿主相关的条件下的黏附和生物膜形成：与大环内酯类耐药性和克隆谱系的关系。
J Med Microbiol. 2019 Feb;68(2):148-160. doi: 10.1099/jmm.0.000893. Epub 2018 Dec 12.
10
Increased biofilm formation by clinical isolates on surfaces covered with plasma proteins.临床分离株在覆盖有血浆蛋白的表面形成生物膜的能力增强。
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001389.

引用本文的文献

1
Dual red and near-infrared LED therapy inhibits MRSA biofilm in otitis media.双红和近红外发光二极管疗法可抑制中耳炎中的耐甲氧西林金黄色葡萄球菌生物膜。
Biofilm. 2025 Aug 21;10:100314. doi: 10.1016/j.bioflm.2025.100314. eCollection 2025 Dec.
2
Proteomic analysis reveals phage-driven metabolic shifts and biofilm disruption in methicillin-resistant Staphylococcus aureus (MRSA).蛋白质组学分析揭示了噬菌体驱动的耐甲氧西林金黄色葡萄球菌（MRSA）的代谢变化和生物膜破坏。
World J Microbiol Biotechnol. 2025 Jun 25;41(7):230. doi: 10.1007/s11274-025-04397-5.
3
Characterization of the Biofilms Formed by Histamine-Producing Strains in the Dairy Environment.

本文引用的文献

1
Sticky Matrix: Adhesion Mechanism of the Staphylococcal Polysaccharide Intercellular Adhesin.黏着基质：葡萄球菌细胞间黏附素的黏附机制。
ACS Nano. 2016 Mar 22;10(3):3443-52. doi: 10.1021/acsnano.5b07515. Epub 2016 Feb 23.
2
Effects of Subinhibitory Concentrations of Ceftaroline on Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilms.头孢洛林亚抑菌浓度对耐甲氧西林金黄色葡萄球菌（MRSA）生物膜的影响。
PLoS One. 2016 Jan 22;11(1):e0147569. doi: 10.1371/journal.pone.0147569. eCollection 2016.
3
Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin.
乳制品环境中组胺产生菌株形成的生物膜的特性分析
Foods. 2023 Apr 3;12(7):1503. doi: 10.3390/foods12071503.
4
Phenotypic and genotypic characterization of linezolid resistance and the effect of antibiotic combinations on methicillin-resistant Staphylococcus aureus clinical isolates.利奈唑胺耐药表型和基因型特征及抗生素联合用药对耐甲氧西林金黄色葡萄球菌临床分离株的影响。
Ann Clin Microbiol Antimicrob. 2023 Apr 3;22(1):23. doi: 10.1186/s12941-023-00574-2.
5
Secreted mammalian DNases protect against systemic bacterial infection by digesting biofilms.分泌型哺乳动物核酸酶通过消化生物膜来抵抗全身细菌感染。
J Exp Med. 2023 Jun 5;220(6). doi: 10.1084/jem.20221086. Epub 2023 Mar 16.
6
Genetic and compositional analysis of biofilm formed by isolated from food contact surfaces.从食品接触表面分离出的生物膜的遗传和成分分析。
Front Microbiol. 2022 Dec 2;13:1001700. doi: 10.3389/fmicb.2022.1001700. eCollection 2022.
7
Genetic Diversity among Methicillin-Resistant in Malaysia (2002-2020).马来西亚耐甲氧西林金黄色葡萄球菌的遗传多样性（2002 - 2020年）
Trop Med Infect Dis. 2022 Nov 8;7(11):360. doi: 10.3390/tropicalmed7110360.
8
Efficacy of celery ( L.) alcoholic extract against systemic methicillin-resistant infection in rat models.芹菜（L.）乙醇提取物对大鼠模型全身性耐甲氧西林感染的疗效。
Vet World. 2022 Apr;15(4):898-905. doi: 10.14202/vetworld.2022.898-905. Epub 2022 Apr 12.
9
Benzimidazolium salts prevent and disrupt methicillin-resistant biofilms.苯并咪唑盐可预防和破坏耐甲氧西林生物膜。
RSC Adv. 2020 Mar 4;10(16):9420-9430. doi: 10.1039/d0ra00738b. eCollection 2020 Mar 2.
10
Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches.利用同步辐射衰减全反射傅里叶变换红外显微光谱学和化学计量学方法分析致病菌和酵母生物膜。
Molecules. 2021 Jun 25;26(13):3890. doi: 10.3390/molecules26133890.
源自小鼠的嗜肺巴斯德氏菌和鼠放线杆菌分离株生物膜形成特性研究
PLoS One. 2015 Oct 2;10(10):e0138778. doi: 10.1371/journal.pone.0138778. eCollection 2015.
4
Antibiotic susceptibility of ica-positive and ica-negative MRSA in different phases of biofilm growth.ica阳性和ica阴性耐甲氧西林金黄色葡萄球菌在生物膜生长不同阶段的抗生素敏感性
J Antibiot (Tokyo). 2015 Jan;68(1):15-22. doi: 10.1038/ja.2014.96. Epub 2014 Jul 30.
5
Fibronectin-binding proteins are required for biofilm formation by community-associated methicillin-resistant Staphylococcus aureus strain LAC.社区相关耐甲氧西林金黄色葡萄球菌菌株LAC形成生物膜需要纤连蛋白结合蛋白。
FEMS Microbiol Lett. 2014 Apr;353(2):157-64. doi: 10.1111/1574-6968.12424. Epub 2014 Apr 10.
6
Applying insights from biofilm biology to drug development - can a new approach be developed?将生物膜生物学的见解应用于药物开发——能否开发出新方法？
Nat Rev Drug Discov. 2013 Oct;12(10):791-808. doi: 10.1038/nrd4000.
7
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) isolates of swine origin form robust biofilms.猪源耐甲氧西林金黄色葡萄球菌（LA-MRSA）分离株形成坚固的生物膜。
PLoS One. 2013 Aug 9;8(8):e73376. doi: 10.1371/journal.pone.0073376. eCollection 2013.
8
Effect of proteases on biofilm formation of the plastic-degrading actinomycete Rhodococcus ruber C208.蛋白酶对塑料降解放线菌红球菌 C208 生物膜形成的影响。
FEMS Microbiol Lett. 2013 May;342(1):18-23. doi: 10.1111/1574-6968.12114. Epub 2013 Mar 18.
9
Dispersal of Bap-mediated Staphylococcus aureus biofilm by proteinase K.蛋白水解酶对 Bap 介导的金黄色葡萄球菌生物膜的分散作用。
J Antibiot (Tokyo). 2013 Feb;66(2):55-60. doi: 10.1038/ja.2012.98. Epub 2012 Nov 14.
10
A semi-quantitative approach to assess biofilm formation using wrinkled colony development.一种使用皱缩菌落发育来评估生物膜形成的半定量方法。
J Vis Exp. 2012 Jun 7(64):e4035. doi: 10.3791/4035.