Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
J Med Microbiol. 2021 Aug;70(8). doi: 10.1099/jmm.0.001389.
Biofilm formation is a major virulence factor associated with infections. However, the influence of plasma proteins on biofilm formation of clinical isolates remains unclear. We hypothesized that coating surfaces with plasma proteins might induce biofilm formation by of different clonal lineages.. To evaluate biofilm production by clinical isolates of different clonal lineages isolated in Rio de Janeiro hospitals and investigated the presence of biofilm-associated genes. This study assessed biofilm production of 60 isolates in polystyrene microtitre plates with and without fibrinogen or fibronectin. The biochemical composition of the biofilm matrices was determined and the biofilm formation on fibrinogen-coated surfaces was also evaluated by confocal laser scanning microscopy. The presence of biofilm-related genes was detected by PCR, and the typing and functionality of was also evaluated. Most of the isolates (45 %) were weak biofilm producers or non-producers. However, most of them presented a significant increase in biofilm production on plates covered with plasma proteins. There was no significant difference in biofilm formation between methicillin-resistant and -susceptible isolates, or between different clonal lineages, except for ST30-IV (weak producers) and ST239-III (strong producers). The gene was associated with higher biofilm production. An increase in biofilm production in the presence of plasma proteins highlights the importance of investigating biofilm formation by clinical isolates under different conditions since this virulence factor contributes to persistent infections and increased resistance to antimicrobials.
生物膜形成是与感染相关的主要毒力因子。然而，血浆蛋白对临床分离株生物膜形成的影响尚不清楚。我们假设通过不同克隆谱系的 来覆盖表面可能会诱导生物膜形成。为了评估在里约热内卢医院分离的不同克隆谱系的临床分离株的生物膜产生情况，并研究生物膜相关基因的存在情况。本研究评估了 60 株临床分离株在聚苯乙烯微量滴定板上有无纤维蛋白原或纤维连接蛋白存在时的生物膜产生情况。通过共焦激光扫描显微镜评估了纤维蛋白原覆盖表面上生物膜形成情况，还测定了生物膜基质的生化组成。通过 PCR 检测生物膜相关基因的存在情况，并对 的分型和功能进行评估。大多数分离株（45%）为弱生物膜生产者或非生产者。然而，它们大多数在覆盖有血浆蛋白的平板上的生物膜产生显著增加。耐甲氧西林和敏感 分离株之间，或不同克隆谱系之间，除了 ST30-IV（弱生产者）和 ST239-III（强生产者）之外，生物膜形成没有显著差异。 基因与更高的生物膜产生有关。在存在血浆蛋白的情况下，生物膜产生增加，突出了在不同条件下研究 临床分离株生物膜形成的重要性，因为这种毒力因子有助于持续感染和增加对抗微生物药物的耐药性。
