Section of Oral Medicine, Department of Head Medicine and Oral Health, University of Leipzig, Liebigstraße 10-14, 04103, Leipzig, Germany.
Department of Oral and Maxillofacial and Facial Plastic Surgery, University of Leipzig, Liebigstraße 10-14, 04103, Leipzig, Germany.
Clin Oral Investig. 2017 Nov;21(8):2493-2498. doi: 10.1007/s00784-017-2047-9. Epub 2017 Jan 11.
The aim of this prospective split sample study was to evaluate the applicability of liquid-based cytology (LBC) of oral brush biopsies for detection of oral cancer.
Two different preparation techniques were investigated: the conventional transfer procedure to glass slides and the LBC preparation method. The obtainments of epithelial cells were performed five times with a nylon brush and transferred onto five glass slides. Additionally, the brushes, which were normally discarded, were stored in a fixative solution. Conventional slides and respective thin layers from a total of 113 cases were reviewed with both techniques.
Thin layers showed excellent morphology on a clear background, which allowed an accurate diagnosis. In contrast, the conventional glass slides showed significantly more blood contamination and cell overlapping. The sensitivity of conventional cytological diagnosis was 96.3%, the specificity archived 90.6%, the positive predictive value was 96.3% and the negative predictive value scored 90.6%. The sensitivity of the cytological diagnosis using thin layers archived 97.5%, the specificity was 68.8%, the positive predictive value revealed 88.76% and negative predictive value was 91.7%.
Our findings indicate that in oral cytology, LBC may replace other types of wet-fixed preparations using the full amount of collected cells, resulting in enhanced specimen quality archiving comparable values of diagnostic accuracy.
LBC facilitates the cell collection due to simpler handling and less transfer errors by dentists and may improve the overall diagnostic accuracy of oral brush biopsies in future.
本前瞻性样本分割研究的目的是评估口腔刷活检液基细胞学（LBC）在口腔癌检测中的适用性。
研究调查了两种不同的制片技术：传统的玻片转移法和 LBC 制片方法。用尼龙刷采集上皮细胞，共采集 5 次，将细胞转移到 5 个玻片上。此外，通常丢弃的刷子被储存在固定液中。用两种技术对总共 113 例的常规玻片和相应的薄层片进行了评估。
薄层片显示出清晰背景下极好的形态，可进行准确诊断。相比之下，传统的玻片显示出明显更多的血液污染和细胞重叠。常规细胞学诊断的敏感性为 96.3%，特异性为 90.6%，阳性预测值为 96.3%，阴性预测值为 90.6%。使用薄层片的细胞学诊断敏感性为 97.5%，特异性为 68.8%，阳性预测值为 88.76%，阴性预测值为 91.7%。
我们的研究结果表明，在口腔细胞学中，LBC 可以替代其他类型的湿固定制剂，使用收集的全部细胞，从而提高标本质量，同时保持诊断准确性相当。
LBC 简化了处理过程，减少了牙医在转移过程中的错误，有助于细胞采集，并可能在未来提高口腔刷活检的整体诊断准确性。
