Sherk Vanessa D, Carpenter R Dana, Giles Erin D, Higgins Janine A, Oljira Robera M, Johnson Ginger C, Mills Samuel, Maclean Paul S
1Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO; 2Department of Mechanical Engineering, University of Colorado Denver, Denver, CO; and 3Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, CO.
Med Sci Sports Exerc. 2017 May;49(5):888-895. doi: 10.1249/MSS.0000000000001194.
Using a nonsteroidal anti-inflammatory drug (NSAID) before a single bout of mechanical loading can reduce bone formation response. It is unknown whether this translates to an attenuation of bone strength and structural adaptations to exercise training.
This study aimed to determine whether nonsteroidal anti-inflammatory drug use before exercise prevents increases in bone structure and strength in response to weight-bearing exercise.
Adult female Wistar rats (n = 43) were randomized to ibuprofen (IBU) or vehicle (VEH) and exercise (EX) or sedentary (SED) groups in a 2 × 2 (drug and activity) ANCOVA design with body weight as the covariate, and data are reported as mean ± SE. IBU drops (30 mg·kg BW) or VEH (volume equivalent) were administered orally 1 h before the bout of exercise. Treadmill running occurred 5 d·wk for 60 min·d at 20 m·min with a 5° incline for 12 wk. Micro-CT, mechanical testing, and finite element modeling were used to quantify bone characteristics.
Drug-activity interactions were not significant. Exercise increased tibia cortical cross-sectional area (EX = 5.67 ± 0.10, SED = 5.37 ± 0.10 mm, P < 0.01) and structural estimates of bone strength (Imax: EX = 5.16 ± 0.18, SED = 4.70 ± 0.18 mm, P < 0.01; SecModPolar: EX = 4.01 ± 0.11, SED = 3.74 ± 0.10 mm, P < 0.01). EX had increased failure load (EX = 243 ± 9, SED = 202 ± 7 N, P < 0.05) and decreased distortion in response to a 200-N load (von Mises stress at tibia-fibula junction: EX = 48.2 ± 1.3, SED = 51.7 ± 1.2 MPa, P = 0.01). There was no effect of ibuprofen on any measurement tested. Femur results revealed similar patterns.
Ibuprofen before exercise did not prevent the skeletal benefits of exercise in female rats. However, exercise that engenders higher bone strains may be required to detect an effect of ibuprofen.
在单次机械负荷前使用非甾体抗炎药（NSAID）可降低骨形成反应。目前尚不清楚这是否会转化为骨强度的减弱以及对运动训练的结构适应性变化。
本研究旨在确定运动前使用非甾体抗炎药是否会阻止负重运动引起的骨结构和强度增加。
成年雌性Wistar大鼠（n = 43）按2×2（药物和活动）协方差分析设计随机分为布洛芬（IBU）组或赋形剂（VEH）组以及运动（EX）组或久坐（SED）组，以体重作为协变量，数据以平均值±标准误表示。在运动前1小时口服IBU滴剂（30mg·kg体重）或VEH（等量体积）。跑步机跑步每周进行5天，每天60分钟，速度为20米/分钟，坡度为5°，持续12周。使用显微CT、力学测试和有限元建模来量化骨特征。
药物 - 活动交互作用不显著。运动增加了胫骨皮质横截面积（EX = 5.67±0.10，SED = 5.37±0.10mm，P < 0.01）以及骨强度的结构估计值（Imax：EX = 5.16±0.18，SED = 4.70±0.18mm，P < 0.01；SecModPolar：EX = 4.01±0.11，SED = 3.74±0.10mm，P < 0.01）。运动使破坏载荷增加（EX = 243±9，SED = 202±7N，P < 0.05），并且在200N负荷下的变形减小（胫腓关节处的冯·米塞斯应力：EX = 48.2±1.3，SED = 51.7±1.2MPa，P = 0.01）。布洛芬对所测试的任何测量指标均无影响。股骨结果显示出相似的模式。
运动前使用布洛芬并未阻止雌性大鼠从运动中获得骨骼益处。然而，可能需要产生更高骨应变的运动才能检测到布洛芬的作用。
