Egea G, Goldstein I J, Roth J
Interdepartmental Electron Microscopy, University of Basel, Switzerland.
Histochemistry. 1989;92(6):515-22. doi: 10.1007/BF00524763.
The Datura stramonium lectin recognizes with high affinity the disaccharide N-acetyllactosamine (Gal beta 1,4 GlcNAc). We have developed a highly specific cytochemical affinity technique in which an ovomucoid-gold complex serves as second step reagent for the visualization of this lectin bound to reactive sequences present in tissue sections. The lectin binding sites were detected in semithin and ultrathin sections of aldehyde-fixed and low temperature Lowicryl K4M embedded tissues. For light microscopical labeling the photochemical silver reaction for signal amplification was required. The application of this technique for the detection of N-acetyllactosamine containing asparagine-linked oligosaccharides in various intracellular organelles and the plasma membrane is demonstrated.
曼陀罗凝集素能高亲和力地识别二糖N-乙酰乳糖胺（半乳糖β1,4 N-乙酰葡糖胺）。我们开发了一种高度特异性的细胞化学亲和技术，其中卵类粘蛋白-金复合物作为第二步试剂，用于可视化与组织切片中存在的反应序列结合的这种凝集素。在醛固定和低温Lowicryl K4M包埋组织的半薄和超薄切片中检测到凝集素结合位点。对于光学显微镜标记，需要进行光化学银反应以放大信号。展示了该技术在检测各种细胞内细胞器和质膜中含N-乙酰乳糖胺的天冬酰胺连接寡糖方面的应用。
