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用凝集素-辣根过氧化物酶结合物对唾液腺和胰腺糖缀合物中糖残基进行光镜检测。I. 小鼠。

Light microscopic detection of sugar residues in glycoconjugates of salivary glands and the pancreas with lectin-horseradish peroxidase conjugates. I. Mouse.

作者信息

Schulte B A, Spicer S S

出版信息

Histochem J. 1983 Dec;15(12):1217-38. doi: 10.1007/BF01002741.

Abstract

Mouse salivary glands and pancreases were stained with a battery of ten horseradish peroxidase-conjugated lectins. Lectin staining revealed striking differences in the structure of oligosaccharides of stored intracellular secretory glycoproteins and glycoconjugates associated with the surface of epithelial cells lining excretory ducts. The percentage of acinar cells containing terminal alpha-N-acetylgalactosamine residues varied greatly in submandibular glands of 30 male mice, but all submandibular acinar cells contained oligosaccharides with terminal sialic acid and penultimate beta-galactose residues. The last named dimer was abundant in secretory glycoprotein of all mucous acinar cells in murine sublingual glands and an additional 20-50% of these cells in all glands contained terminal N-acetylglucosamine residues. In contrast, terminal alpha-N-acetylgalactosamine was abundant in sublingual serous demilune secretions. Serous acinar cells in the exorbital lacrimal gland, posterior lingual gland, parotid gland and pancreas exhibited a staining pattern unique to each organ. In contrast, the apical cytoplasm and surface of striated duct epithelial cells in the submandibular, sublingual, parotid and exorbital lacrimal gland stained similarly. A comparison of staining with conjugated lectins reported biochemically to have very similar carbohydrate binding specificity has revealed some remarkable differences in their reactivity, suggesting different binding specificity for the same terminal sugars having different glycosidic linkages or with different penultimate sugar residues.

摘要

用一组十种辣根过氧化物酶偶联凝集素对小鼠唾液腺和胰腺进行染色。凝集素染色显示,储存的细胞内分泌糖蛋白和与排泄管内衬上皮细胞表面相关的糖缀合物的寡糖结构存在显著差异。在30只雄性小鼠的下颌下腺中,含有末端α-N-乙酰半乳糖胺残基的腺泡细胞百分比差异很大,但所有下颌下腺泡细胞都含有带有末端唾液酸和倒数第二个β-半乳糖残基的寡糖。上述二聚体在小鼠舌下腺所有黏液腺泡细胞的分泌糖蛋白中含量丰富,所有腺体中另外20%-50%的这些细胞含有末端N-乙酰葡糖胺残基。相比之下,末端α-N-乙酰半乳糖胺在舌下腺浆液半月分泌中含量丰富。眶外泪腺、舌后腺、腮腺和胰腺中的浆液腺泡细胞呈现出每个器官特有的染色模式。相比之下,下颌下腺、舌下腺、腮腺和眶外泪腺中纹状管上皮细胞的顶端细胞质和表面染色相似。对生化报道具有非常相似碳水化合物结合特异性的偶联凝集素染色进行比较,发现它们的反应性存在一些显著差异,这表明对具有不同糖苷键或不同倒数第二个糖残基的相同末端糖具有不同的结合特异性。

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