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单次气管内给予纳米材料后小鼠肺中DNA甲基化的变化

Changes in DNA Methylation in Mouse Lungs after a Single Intra-Tracheal Administration of Nanomaterials.

作者信息

Tabish Ali M, Poels Katrien, Byun Hyang-Min, Luyts Katrien, Baccarelli Andrea A, Martens Johan, Kerkhofs Stef, Seys Sven, Hoet Peter, Godderis Lode

机构信息

Centre for Environment and Health, KU Leuven, Leuven, Belgium.

Integrated Cardio Metabolic Centre, Huddinge, Sweden.

出版信息

PLoS One. 2017 Jan 12;12(1):e0169886. doi: 10.1371/journal.pone.0169886. eCollection 2017.

DOI:10.1371/journal.pone.0169886
PMID:28081255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5231360/
Abstract

AIMS

This study aimed to investigate the effects of nanomaterial (NM) exposure on DNA methylation.

METHODS AND RESULTS

Intra-tracheal administration of NM: gold nanoparticles (AuNPs) of 5-, 60- and 250-nm diameter; single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) at high dose of 2.5 mg/kg and low dose of 0.25 mg/kg for 48 h to BALB/c mice. Study showed deregulations in immune pathways in NM-induced toxicity in vivo. NM administration had the following DNA methylation effects: AuNP 60 nm induced CpG hypermethylation in Atm, Cdk and Gsr genes and hypomethylation in Gpx; Gsr and Trp53 showed changes in methylation between low- and high-dose AuNP, 60 and 250 nm respectively, and AuNP had size effects on methylation for Trp53.

CONCLUSION

Epigenetics may be implicated in NM-induced disease pathways.

摘要

目的

本研究旨在调查纳米材料（NM）暴露对DNA甲基化的影响。

方法与结果

对BALB/c小鼠进行气管内给予纳米材料：直径为5纳米、60纳米和250纳米的金纳米颗粒（AuNP）；高剂量2.5毫克/千克和低剂量0.25毫克/千克的单壁碳纳米管（SWCNT）和多壁碳纳米管（MWCNT），持续48小时。研究表明，在体内纳米材料诱导的毒性中免疫途径失调。纳米材料给药具有以下DNA甲基化效应：60纳米的AuNP诱导Atm、Cdk和Gsr基因中的CpG高甲基化以及Gpx中的低甲基化；Gsr和Trp53分别在低剂量和高剂量的60纳米及250纳米AuNP之间显示出甲基化变化，并且AuNP对Trp53的甲基化具有尺寸效应。

结论

表观遗传学可能与纳米材料诱导的疾病途径有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/61e838f6bb49/pone.0169886.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/ad4a51112297/pone.0169886.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f5e400734f3d/pone.0169886.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f1a54a9ec0a6/pone.0169886.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f7aba9ac3cd6/pone.0169886.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/61e838f6bb49/pone.0169886.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/ad4a51112297/pone.0169886.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f5e400734f3d/pone.0169886.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f1a54a9ec0a6/pone.0169886.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/f7aba9ac3cd6/pone.0169886.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df2e/5231360/61e838f6bb49/pone.0169886.g005.jpg

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