a Centro de Investigación en Enfermedades Infecciosas , Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas , Ciudad de México , Mexico.
Ocul Immunol Inflamm. 2018;26(1):74-81. doi: 10.1080/09273948.2016.1268170. Epub 2017 Jan 12.
To characterize the immunologic profile in aqueous humor (AqH) of HIV-infected individuals with cytomegalovirus retinitis (CMVr) or ocular syphilis and to assess if AqH and plasma represent independent cytokine compartments.
Concentrations of 27 cytokines in AqH and plasma of HIV-infected individuals with CMVr (n = 23) or ocular syphilis (n = 16) were measured by multiplex assay. Cytokine profiles of both groups were compared.
Individuals with CMVr had higher plasma concentrations of interleukin (IL)-7, IL-8, IL-10, interferon (IFN)-γ, IFN-α2, G-CSF, IP-10 and IL-1α; as well as higher AqH concentrations of IL-1α, IP-10 and GM-CSF than those with ocular syphilis. AqH and plasma levels correlated only for IP-10 in both ocular infections.
Individuals with CMVr had higher plasma cytokine levels than those with ocular syphilis. The immunologic profiles in AqH and plasma are independent. Therefore, AqH cytokine concentrations cannot be inferred from plasma cytokine concentrations in the population studied.
分析人类免疫缺陷病毒（HIV）感染合并巨细胞病毒视网膜炎（CMVr）或眼梅毒患者房水中的免疫特征，并评估房水和血浆是否代表独立的细胞因子隔室。
采用多重分析方法检测 23 例合并 CMVr 和 16 例眼梅毒的 HIV 感染患者房水和血浆中 27 种细胞因子的浓度。比较两组患者的细胞因子谱。
与眼梅毒患者相比，CMVr 患者的血浆中白细胞介素（IL）-7、IL-8、IL-10、干扰素（IFN）-γ、IFN-α2、G-CSF、IP-10 和 IL-1α浓度更高；房水中的 IL-1α、IP-10 和 GM-CSF 浓度也更高。在两种眼部感染中，房水和血浆水平仅与 IP-10 相关。
CMVr 患者的血浆细胞因子水平高于眼梅毒患者。房水和血浆中的免疫特征是独立的。因此，在研究人群中，不能从血浆细胞因子浓度推断房水细胞因子浓度。
