眼表疾病患者眼内房水细胞因子水平升高。

Elevated Aqueous Cytokine Levels in Eyes With Ocular Surface Diseases.

作者信息

Aketa Naohiko, Yamaguchi Takefumi, Asato Teru, Yagi-Yaguchi Yukari, Suzuki Terumasa, Higa Kazunari, Kurihara Toshihide, Satake Yoshiyuki, Tsubota Kazuo, Shimazaki Jun

机构信息

Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.

出版信息

Am J Ophthalmol. 2017 Dec;184:42-51. doi: 10.1016/j.ajo.2017.09.029. Epub 2017 Oct 5.

DOI:10.1016/j.ajo.2017.09.029

PMID:28988898

Abstract

PURPOSE

To evaluate cytokine and protein levels in the aqueous humor (AqH) of eyes with ocular surface diseases.

DESIGN

Prospective consecutive case series.

METHODS

This study includes 14 patients (aged 62.4 ± 13.7 years) with chronic-phase ocular surface diseases (4 with ocular cicatricial pemphigoid, 5 with chemical burns, 2 with a thermal burn, 2 with Stevens-Johnson syndrome, and 1 with exposure keratitis), 14 matched patients without ocular surface disease (controls with corneal scar), and 30 patients who underwent cataract surgery (healthy controls). AqH samples were collected at the beginning of surgery. AqH levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, monocyte chemotactic protein [MCP]-1, interferon [IFN]-α, IFN-γ, macrophage inflammatory protein [MIP]-1α, MIP-1β, P-selectin, E-selectin, soluble-intercellular adhesion molecule [s-ICAM]-1, tumor necrosis factor [TNF]-α, granulocyte-macrophage colony-stimulating factor [GM-CSF], IFN-γ-induced protein [IP]-10) were measured using multiplex beads immunoassays.

RESULTS

The levels of IL-6, IL-10, IL-17A, GM-CSF, E-selectin, P-selectin, and s-ICAM in AqH were significantly elevated in eyes with ocular surface diseases (in pg/mL: 1696 ± 804, 4.0 ± 1.0, 24.3 ± 9.8, 26.0 ± 18.3, 5150 ± 1232, 13122 ± 7219, and 7914 ± 2813, respectively), compared to healthy controls (IL-6: 6.36 ± 0.94, P = .001; IL-10: 1.68 ± 0.04, P = .0006; IL-17A: 3.7 ± 0.2, P = .008; GM-CSF: 2.7 ± 0.3, P = .007; E-selectin: 2093 ± 37, P = .0001; P-selectin: 3658 ± 137, P = .0001; sICAM-1: 1397 ± 119, P = .008). The levels of IL-6, IL-17A, E-selectin, and P-selectin in AqH were significantly higher in eyes with ocular surface diseases compared to those with corneal scar (IL-6: 44.1 ± 15.0, P = .0077; IL-17A: 4.1 ± 0.7, P = .034; E-selectin: 2439 ± 302, P = .039; and P-selectin: 5673 ± 1553, P = .017).

CONCLUSIONS

Multiple AqH cytokine levels were elevated in chronic ocular surface diseases.

摘要

目的

评估眼表疾病患者房水中的细胞因子和蛋白质水平。

设计

前瞻性连续病例系列研究。

方法

本研究纳入14例慢性期眼表疾病患者（年龄62.4±13.7岁）（4例瘢痕性类天疱疮、5例化学伤、2例热烧伤、2例史蒂文斯-约翰逊综合征、1例暴露性角膜炎）、14例匹配的无眼表疾病患者（角膜瘢痕对照）和30例行白内障手术的患者（健康对照）。在手术开始时采集房水样本。使用多重微珠免疫测定法测量房水中细胞因子（白细胞介素[IL]-1α、IL-1β、IL-4、IL-6、IL-8、IL-10、IL-12p70、IL-13、IL-17A、单核细胞趋化蛋白[MCP]-1、干扰素[IFN]-α、IFN-γ、巨噬细胞炎性蛋白[MIP]-1α、MIP-1β、P-选择素、E-选择素、可溶性细胞间黏附分子[s-ICAM]-1、肿瘤坏死因子[TNF]-α、粒细胞-巨噬细胞集落刺激因子[GM-CSF]、IFN-γ诱导蛋白[IP]-10）的水平。

结果

与健康对照相比，眼表疾病患者房水中IL-6、IL-10、IL-十七A、GM-CSF、E-选择素、P-选择素和s-ICAM水平显著升高（单位：pg/mL，分别为：1696±804、4.0±1.0、24.3±9.8, 26.0±18.3, 5150±1232, 13122±7219, 7914±2813）（IL-6：6.36±0.94，P = 0.001；IL-10：1.68±0.04，P = 0.0006；IL-17A：3.7±0.2，P = 0.008；GM-CSF：2.7±0.3，P = 0.007；E-选择素：2093±37，P = 0.0001；P-选择素：3658±137，P = 0.0001；sICAM-1：1397±119，P = 0.008）。与角膜瘢痕患者相比，眼表疾病患者房水中IL-6、IL-17A、E-选择素和P-选择素水平显著更高（IL-6：44.1±15.0，P = 0.0077；IL-17A：4.1±0.7，P = 0.034；E-选择素：2439±302，P = 0.039；P-选择素：5673±1553，P = 0.017）。