Kong W J, Guo C G, Xie L Y, Wei W B, Dong H W, Chen C, Du K F
Department of Ophthalmology, Beijing You'an Hospital, Capital Medical University, Beijing 100069, China.
Zhonghua Yan Ke Za Zhi. 2017 Oct 11;53(10):746-752. doi: 10.3760/cma.j.issn.0412-4081.2017.10.006.
To explore the Cytokine of aqueous humor in AIDS patients with different eye diseases. A case-control study including 38 patients who was diagnosed as acquired immunodeficiency syndrome(AIDS) at Infectious Center and Department of Ophthalmology in Beijing You'an Hospital, Capital Medical University. And 16 cases of 38 patients were cytomegalovirus retinitis (CMVR), 14 cases were HIV-related retinal disease, and 8 cases presented as normal. A series of tests were performed including vision acuity check, intraocular pressure, fundus photography, blood cell count of CD4+ T lymphocyte and the content of cytokines in aqueous humor by Luminex 200(TM) liquid chip analyzer. Intraocular pressure was analyzed by one-way ANOVA, visual acuity, CD4(+) T lymphocyte count and cytokines were analyzed by Kruskal-Wallis test, the differences between the two groups were compared by Mann-Whitney test corrected with Bonferroni. Of the 38 AIDS patients [mean age (33.7±10.0) year], 37 were males and 1 was female. The mean visual acuity of 38 patients is logMAR 1.00(0.000, 1.000). The difference of visual acuity among three groups was statistically significant (χ(2)=9.963, 0.007). The visual acuity in CMVR group was significantly higher than that in HIV-related retinal disease group and AIDS-normal eye group. There was no statistically significant difference of intraocular pressure among three groups (=0.830, <0.05). The mean CD4+ T lymphocyte count was 58(4, 550)/μl and the difference was statistically significant among three groups(χ(2)=6.106, 0.047). The CD4+ T lymphocyte count in AIDS-normal eye group was significantly higher than that in CMVR group and HIV-related retinal disease group. There was no statistically significant difference in CD4+ T lymphocyte count between HIV-related retinal disease group and CMVR group. The following cytokines in CMVR group were significantly higher than the other two groups with statistically significant differences: MIP-1b, IL-6, TNF-α, VEGF, IL-8, MCP-1, IP-10. In patients who was diagnosed as AIDS, cytomegalovirus retinitis would damage vision acuity seriously. The elevated cytokines' level in aqueous humor such as MIP-1b, IL-6, TNF-α, VEGF, IL-8, MCP-1, IP-10, compared with HIV-related retinal disease and AIDS-normal eye cases, may be related to the activity of Th1 cell and monocyte-macrophages system including chemokines, inflammatory factors, and vascular endothelial factors. .
探讨不同眼部疾病艾滋病患者房水中的细胞因子。一项病例对照研究，纳入了38例在首都医科大学附属北京佑安医院感染科和眼科被诊断为获得性免疫缺陷综合征（AIDS）的患者。38例患者中16例为巨细胞病毒性视网膜炎（CMVR），14例为HIV相关视网膜疾病，8例表现正常。进行了一系列检查，包括视力检查、眼压测量、眼底照相、CD4 + T淋巴细胞血细胞计数以及使用Luminex 200（TM）液体芯片分析仪检测房水中细胞因子的含量。眼压采用单因素方差分析，视力、CD4（+）T淋巴细胞计数和细胞因子采用Kruskal - Wallis检验，两组间差异采用Bonferroni校正的Mann - Whitney检验进行比较。38例艾滋病患者[平均年龄（33.7±10.0）岁]，男性37例，女性1例。38例患者的平均视力为logMAR 1.00（0.000，1.000）。三组间视力差异有统计学意义（χ（2）=9.963，P = 0.007）。CMVR组的视力显著高于HIV相关视网膜疾病组和艾滋病正常眼组。三组间眼压无统计学差异（F = 0.830，P>0.05）。CD4 + T淋巴细胞平均计数为58（4，550）/μl，三组间差异有统计学意义（χ（2）=6.106，P = 0.047）。艾滋病正常眼组的CD4 + T淋巴细胞计数显著高于CMVR组和HIV相关视网膜疾病组。HIV相关视网膜疾病组和CMVR组之间的CD4 + T淋巴细胞计数无统计学差异。CMVR组中以下细胞因子显著高于其他两组，差异有统计学意义：MIP - 1b、IL - 6、TNF - α、VEGF、IL - 8、MCP - 1、IP - 10。在被诊断为艾滋病的患者中，巨细胞病毒性视网膜炎会严重损害视力。与HIV相关视网膜疾病和艾滋病正常眼病例相比，房水中MIP - 1b、IL - 6、TNF - α、VEGF、IL - 8、MCP - 1、IP - 10等细胞因子水平升高，可能与包括趋化因子、炎症因子和血管内皮因子在内的Th1细胞和单核巨噬细胞系统的活性有关。
