Swanson G J, Nomura S, Hogan B L
Imperial Cancer Research Fund, Department of Zoology, Oxford, U.K.
Hear Res. 1989 Sep;41(2-3):169-77. doi: 10.1016/0378-5955(89)90008-7.
Using in situ hybridisation we have determined the distribution of expression of 2ar (also known as osteopontin, bone sialoprotein 1 or 44-kDa bone phosphoprotein) in the developing mouse inner ear. We have identified several discrete sites, both osteogenic and non-osteogenic, that express 2ar from embryonic day 16.5 (E16.5). In addition to the regions of developing bone of the calvaria and temporal bone, we have found 2ar expression in the epithelium of the sensory maculae (but not in the organ of Corti), in the vestibular and auditory ganglia and nerves (but not in the nerves that innervate the whiskers in the snout), in the epithelium that lines the endolymphatic sac (but not in the neighbouring and contiguous endolymphatic duct) and also in the epithelium that lines the semicircular canals. We found also individual cells scattered throughout the brain, loose mesenchyme and blood vessels of the head that were expressing 2ar. Several of the sites in the inner ear, for example the maculae and the endolymphatic sac, are known to be involved in the production of calcified matrix. The results extend the range of tissue types known to express the protein and demonstrate that tissues of histologically similar appearance can nonetheless differ in their gene expression.
我们利用原位杂交技术确定了2ar（也称为骨桥蛋白、骨唾液蛋白1或44 kDa骨磷蛋白）在发育中的小鼠内耳中的表达分布。我们鉴定出了几个离散的位点，包括成骨和非成骨位点，它们从胚胎第16.5天（E16.5）开始表达2ar。除了颅盖骨和颞骨的发育骨区域外，我们还发现2ar在前庭斑上皮（但不在柯蒂氏器中）、前庭和听觉神经节及神经（但不在支配口鼻部触须的神经中）、内淋巴囊内衬上皮（但不在相邻的内淋巴管中）以及半规管内衬上皮中表达。我们还发现个别细胞散布在整个大脑、头部的疏松间充质和血管中，这些细胞表达2ar。内耳中的几个位点，例如前庭斑和内淋巴囊，已知参与钙化基质的产生。这些结果扩展了已知表达该蛋白的组织类型范围，并表明组织学外观相似的组织在基因表达上仍可能存在差异。
