Ge Hongyan, Zou Di, Wang Yingshu, Jiang Hao, Wang Liping
Department of Gastroenterology, Affiliated Hospital of Inner Mongolia University for the Nationalities/Clinical Medicine College of Inner Mongolia University for the NationalitiesTongliaoP.R. China.
Department of Nephrology, The First Affiliated Hospital to Changchun University of Chinese MedicineChangchunP.R. China.
Oncol Res. 2017 Jan 2;25(1):29-34. doi: 10.3727/096504016X14719078133168.
Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3'-untranslated region (3'-UTR). Functionally, knockdown of miR-377 noticeably increased HCC cell growth and colony formation and inhibited apoptosis. In contrast, overexpression of miR-377 suppressed cell proliferation and increased apoptosis. This study provides new insights for the use of miR-377 as a potential molecular target in HCC therapy.
异常表达的微小RNA（miRNA/miR）及其在癌症发展中的作用最近受到了更多关注。然而，miRNA在肝细胞癌（HCC）中的潜在作用在很大程度上仍不清楚。在本研究中，我们证明miR-377在肝癌细胞系和原发性人类肝癌组织中显著下调。miR-377表达的降低通过靶向Bcl-xL的3'-非翻译区（3'-UTR）导致其表达上调。在功能上，敲低miR-377显著增加了肝癌细胞的生长和集落形成，并抑制了细胞凋亡。相反，miR-377的过表达抑制了细胞增殖并增加了细胞凋亡。本研究为将miR-377用作肝癌治疗的潜在分子靶点提供了新的见解。
