miR-3150b 通过靶向 GOLPH3 抑制肝癌细胞增殖、迁移和侵袭。
MiR-3150b inhibits hepatocellular carcinoma cell proliferation, migration and invasion by targeting GOLPH3.
机构信息
Department of General Surgery, Xi'an XD Group Hospital, Xi'an, China.
Department of Gastroenterology, Xi'an XD Group Hospital, Xi'an, China
出版信息
J Investig Med. 2020 Mar;68(3):770-775. doi: 10.1136/jim-2019-001181. Epub 2019 Dec 5.
BACKGROUND
In this study, we aimed to explore the potential involvement of miR-3150b in hepatocellular carcinoma (HCC) carcinogenesis.
METHODS
The expression of miR-3150b and Golgi phosphoprotein 3 (GOLPH3) was determined in HCC cell lines. Cell proliferation, migration and invasion were estimated by Cell Counting Kit-8, wound healing and Transwell assays. The association between miR-3150b and GOLPH3 was verified by luciferase assay.
RESULTS
MiR-3150b was downregulated, while GOLPH3 was remarkably upregulated in HCC cells. Furthermore, miR-3150b inhibited HCC cell proliferation, migration and invasion. MiR-3150b directly targeted and negatively regulated GOLPH3.
CONCLUSION
MiR-3150b suppressed HCC cell proliferation, invasion and migration by targeting GOLPH3.
背景
本研究旨在探讨 miR-3150b 在肝细胞癌（HCC）发生发展中的潜在作用。
方法
检测 miR-3150b 和高尔基磷蛋白 3（GOLPH3）在 HCC 细胞系中的表达。通过细胞计数试剂盒-8 检测、划痕愈合实验和 Transwell 实验评估细胞增殖、迁移和侵袭。通过荧光素酶报告实验验证 miR-3150b 和 GOLPH3 之间的关联。
结果
miR-3150b 在 HCC 细胞中下调，而 GOLPH3 则显著上调。此外，miR-3150b 抑制 HCC 细胞的增殖、迁移和侵袭。miR-3150b 可以直接靶向并负调控 GOLPH3。
结论
miR-3150b 通过靶向 GOLPH3 抑制 HCC 细胞的增殖、侵袭和迁移。
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