Grey P A, Kirov S M
Department of Pathology, University of Tasmania Clinical School, Hobart, Australia.
Epidemiol Infect. 1993 Apr;110(2):279-87. doi: 10.1017/s0950268800068217.
Aeromonas strains (total = 60) of clinical, water and food origin were tested for adherence to HEp-2 cells. Environmental strains were selected (except for A. caviae) to include primarily those expressing other virulence-associated properties. Adhesion was markedly species-dependent (A. veronii biotype sobria, 15 of 26 [58%]. A caviae, 4 of 12 [33%] and A. hydrophila, 2 of 8 [11%]). A. veronii biotype sobria were adhesive, irrespective of source (62 and 54% for clinical and environmental strains, respectively). Adherent strains of this species were enterotoxin-positive and most (13 of 15) grew at 43 degrees C. A. caviae isolated from clinical specimens contained a higher proportion (75%) of adherent strains than environmental strains (13%). Virulent subsets of A. veronii biotype sobria and A. caviae are adherent to HEp-2 cells. The HEp-2 assay is a useful model for investigating mechanisms of adherence and enteropathogenicity of virulent Aeromonas species.
对来自临床、水和食物的60株气单胞菌菌株进行了黏附HEp - 2细胞的检测。选择环境菌株（除豚鼠气单胞菌外）主要是那些表达其他毒力相关特性的菌株。黏附明显具有种属依赖性（维罗纳气单胞菌温和生物型，26株中有15株[58%]；豚鼠气单胞菌，12株中有4株[33%]；嗜水气单胞菌，8株中有2株[33%]）。维罗纳气单胞菌温和生物型具有黏附性，与来源无关（临床菌株和环境菌株的黏附率分别为62%和54%）。该种的黏附菌株为肠毒素阳性，且大多数（15株中的13株）能在43℃生长。从临床标本中分离出的豚鼠气单胞菌中黏附菌株的比例（75%）高于环境菌株（13%）。维罗纳气单胞菌温和生物型和豚鼠气单胞菌的有毒亚群可黏附于HEp - 2细胞。HEp - 2检测是研究有毒气单胞菌黏附机制和肠道致病性的有用模型。
