巴西牛群中出现神经综合征的恶性卡他热
Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome.
作者信息
Martins Maira de S N, Castro Alessandra M M G de, Lima Michele Dos S, Pinto Vivian da S C, Silva Thaís G da, Fava Claudia Del, Depes Claudio Regis, Okuda Liria H, Pituco Edviges M
机构信息
Instituto Biológico, Laboratório de Viroses de Bovídeos, São Paulo, SP, Brazil.
Complexo Educacional Faculdade Metropolitanas Unidas, São Paulo, SP, Brazil.
出版信息
Braz J Microbiol. 2017 Apr-Jun;48(2):366-372. doi: 10.1016/j.bjm.2016.10.021. Epub 2017 Jan 2.
Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 10DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 10DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.
对患有神经综合征的牛的中枢神经系统进行了恶性卡他热（MCF）调查。通过组织学分析了290个样本，并优化和验证了检测2型绵羊疱疹病毒（OvHV-2）的分子方法。定性聚合酶链反应（定性PCR）的分析灵敏度为10个DNA拷贝/μL，发现OvHV-2阳性率为4.8%（14/290）。定量聚合酶链反应（qPCR）的分析灵敏度为1个DNA拷贝/μL，阳性率为5.9%（17/290），其中47.1%（8/17）的阳性样本呈现非化脓性脑膜脑炎的组织学证据。对定性PCR产物（ORF75区域的422bp）进行测序并进行系统发育分析。同一性矩阵显示本研究中获得的OvHV-2样本与从GenBank中检索到的样本具有100%的相似性，并证实了其他研究。
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