表型微阵列分析在抗真菌药物研发中的应用。
The application of phenotypic microarray analysis to anti-fungal drug development.
作者信息
Greetham Darren, Lappin David F, Rajendran Ranjith, O'Donnell Lindsay, Sherry Leighann, Ramage Gordon, Nile Christopher
机构信息
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK.
Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, UK.
出版信息
J Microbiol Methods. 2017 Mar;134:35-37. doi: 10.1016/j.mimet.2017.01.004. Epub 2017 Jan 9.
Candida albicans metabolic activity in the presence and absence of acetylcholine was measured using phenotypic microarray analysis. Acetylcholine inhibited C. albicans biofilm formation by slowing metabolism independent of biofilm forming capabilities. Phenotypic microarray analysis can therefore be used for screening compound libraries for novel anti-fungal drugs and measuring antifungal resistance.
利用表型微阵列分析测定了白色念珠菌在有和没有乙酰胆碱情况下的代谢活性。乙酰胆碱通过减缓代谢抑制白色念珠菌生物膜形成，且与生物膜形成能力无关。因此，表型微阵列分析可用于筛选化合物文库以寻找新型抗真菌药物并测定抗真菌耐药性。
Zotero 插件