Kawamoto T, Nishi M, Takahashi K, Nishiyama T, Sato J D, Taniguchi S
Dept. of Biochemistry, Okayama University Dental School, Japan.
In Vitro Cell Dev Biol. 1989 Oct;25(10):965-70. doi: 10.1007/BF02624011.
The stimulatory effects of transforming growth factor beta (TGF-beta) on epidermal growth factor (EGF)-dependent growth of adult and newborn human fibroblasts were investigated. EGF-stimulated growth in low serum of dermal fibroblasts from a 41 year-old adult (HSF-41) was less than half that of newborn foreskin fibroblasts (HFF). The EGF-stimulated growth of HFF after 55 population doublings (HFF-55) was similarly reduced. The decreased growth response to EGF of fibroblasts aged in vivo and in vitro appeared to result principally from a decreased sensitivity to EGF due to a decreased number and affinity of high affinity EGF receptors (H-EGFR). Pre-incubation of HSF-41 and HFF-55 with 25 pM TGF-beta enhanced the growth responses of these cells to EGF and increased the levels of high affinity EGF-binding by these cells. Thus, the stimulation by TGF-beta of EGF-dependent growth of human fibroblasts aged in vivo or in vitro is mediated by increased levels of high affinity EGF binding.
研究了转化生长因子β（TGF-β）对成人及新生儿人成纤维细胞依赖表皮生长因子（EGF）生长的刺激作用。来自一名41岁成年人的真皮成纤维细胞（HSF-41）在低血清中受EGF刺激的生长速度不到新生儿包皮成纤维细胞（HFF）的一半。HFF在经历55次群体倍增后（HFF-55）受EGF刺激的生长同样降低。体内和体外老化的成纤维细胞对EGF的生长反应降低似乎主要是由于高亲和力EGF受体（H-EGFR）数量和亲和力降低导致对EGF的敏感性降低。用25 pM TGF-β预孵育HSF-41和HFF-55可增强这些细胞对EGF的生长反应，并增加这些细胞的高亲和力EGF结合水平。因此，TGF-β对体内或体外老化的人成纤维细胞依赖EGF生长的刺激是通过增加高亲和力EGF结合水平来介导的。
