Plisko A, Gilchrest B A
J Gerontol. 1983 Sep;38(5):513-8. doi: 10.1093/geronj/38.5.513.
A sensitive culture system was used to investigate whether in vitro senescence is associated with loss of responsiveness to relevant growth factors in the cellular environment. Early passage dermal fibroblasts obtained from healthy newborns and adults were maintained 4 to 6 days in medium containing either fetal calf serum or five defined growth factors. Newborn fibroblasts consistently grew far better than adult fibroblasts in both media; dose-response curves demonstrated a greater response by newborn fibroblasts to both serum mitogens and to thrombin, insulin, and epidermal growth factor alone and in combination. These data suggest that newborn fibroblasts generally have greater mitogenic responsiveness than adult fibroblasts and that age-associated loss of growth factor responsiveness may indeed contribute to the decreased proliferative capacity of old cells.
采用一种敏感的培养系统来研究体外衰老是否与细胞环境中对相关生长因子反应性的丧失有关。从健康新生儿和成人获取的早期传代真皮成纤维细胞在含有胎牛血清或五种特定生长因子的培养基中培养4至6天。在这两种培养基中，新生儿成纤维细胞的生长始终远优于成人成纤维细胞；剂量反应曲线表明，新生儿成纤维细胞对血清促有丝分裂原以及单独和联合使用的凝血酶、胰岛素和表皮生长因子的反应更强。这些数据表明，新生儿成纤维细胞通常比成人成纤维细胞具有更高的有丝分裂反应性，并且与年龄相关的生长因子反应性丧失可能确实导致了衰老细胞增殖能力的下降。
