Zhang Huan, Zheng Yi, Wu Yonggen, Ye Danna, Huang Xuefeng
Huan Zhang, MD, Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.
Yi Zheng, MD, Department of Reproductive Medical Center, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.
Pak J Med Sci. 2016 Nov-Dec;32(6):1375-1379. doi: 10.12669/pjms.326.10809.
To investigate whether early embryo cleavage kinetics were affected by type of culture media.
In this prospective sibling-split study, 620 oocytes from 37 patients were randomly allocated into two groups: Cook group and Vitrolife group. Oocytes/embryos in Cook group, would be cultured with Cook sequential culture medium, while oocytes/embryos in Vitrolife group, would be cultured with Vitrolife sequential culture medium. Time-lapse imaging technology was used to calculate exact timing of early embryo cleavage events which included time to 2PN breakdown, cleavage to 2-, 3-, 4-, 5- cell and the time duration in the 2-,3-cell stage. Then these timing of early embryo cleavage events were compared between Cook group and Vitrolife group. Moreover, fertilization rate, cleavage rate, high quality embryo rate, usable blastocyst rate, pregnancy rate and implantation rate of these two groups were also analyzed.
The results showed there were no differences in all timing of early embryo cleavage events between the two groups. In addition, the two groups were similar in fertilization rate (Cook 71.0% vs. Vitrolife 71.3%, P>0.05), cleavage rate (Cook 98.1% vs. Vitrolife 98.2%, P>0.05), high quality embryo rate (Cook 52.1% vs. Vitrolife 52.7%, P>0.05), usable blastocyst rate (Cook 29.7% vs. Vitrolife 28.0%, P>0.05), pregnancy rate (Cook 46.7% VS. Vitrolife 50.0%, P>0.05) and implantation rate (Cook 30.3% VS. Vitrolife 29.0%, P>0.05).
Morphokinetics used for embryo selection are not affected by the two different culture media.
研究早期胚胎分裂动力学是否受培养基类型的影响。
在这项前瞻性同胞卵裂球分割研究中，将37例患者的620个卵母细胞随机分为两组：库克组和Vitrolife组。库克组的卵母细胞/胚胎用库克序贯培养基培养，而Vitrolife组的卵母细胞/胚胎用Vitrolife序贯培养基培养。采用延时成像技术计算早期胚胎分裂事件的精确时间，包括2PN崩解时间、分裂为2、3、4、5细胞的时间以及在2、3细胞阶段的持续时间。然后比较库克组和Vitrolife组早期胚胎分裂事件的这些时间。此外，还分析了两组的受精率、卵裂率、优质胚胎率、可用囊胚率、妊娠率和着床率。
结果显示，两组早期胚胎分裂事件的所有时间均无差异。此外，两组在受精率（库克组71.0%对Vitrolife组71.3%，P>0.05）、卵裂率（库克组98.1%对Vitrolife组98.2%，P>0.05）、优质胚胎率（库克组52.1%对Vitrolife组52.7%，P>0.05）、可用囊胚率（库克组29.7%对Vitrolife组28.0%，P>0.05）、妊娠率（库克组46.7%对Vitrolife组50.0%，P>0.05）和着床率（库克组30.3%对Vitrolife组29.0%，P>0.05）方面相似。
用于胚胎选择的形态动力学不受两种不同培养基的影响。
