Shekhawat Mahipal S, Manokari M
Department of Plant Science, M.G.G.A.C., Mahe, Pondicherry, India.
Department of Botany, K.M. Centre for Postgraduate Studies, Pondicherry, India.
Scientifica (Cairo). 2016;2016:6587571. doi: 10.1155/2016/6587571. Epub 2016 Dec 19.
The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds' induction and their growth were observed with -Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of were rooted and shoots were induced when pretreated with 400 mg L NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg Lindole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg Lindole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.
本研究探讨了外源生长素对一种濒危树木（纳加林甘姆）茎段不定芽和不定根发育的潜力。在温室中进行了实验，以评估不同浓度生长素对茎段插穗茎和根形态特征的影响。在所测试的生长素中，用α-萘乙酸（NAA）处理的节段插穗对芽诱导数量及其生长有显著影响。当用400毫克/升NAA预处理5分钟时，该植物100%的茎段插穗生根并诱导出芽。79%的茎段插穗对300毫克/升吲哚-3-丁酸（IBA）预处理5分钟有反应，75%的茎段插穗用400毫克/升吲哚-3-乙酸（IAA）诱导出芽。发现茎段插穗上至少有5个节是根和芽诱导的先决条件。从茎段插穗培育的植株在自然土壤条件下约92%存活。这是关于通过茎段插穗对该植物进行营养繁殖的首次报道，可用于该濒危药用树木的保护策略和可持续利用。
