Zong Shuai, Li Lan, Li Jinglei, Shaikh Farnaz, Yang Liu, Ye Ming
College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
Appl Biochem Biotechnol. 2017 Jun;182(2):669-686. doi: 10.1007/s12010-016-2353-8. Epub 2017 Jan 12.
In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as CHNOS. The possible chemical structure of LIM205 was determined according to the results of Fourier transform infrared (FT-IR), H NMR, C NMR, and pyrolysis/GC-MS analysis. With the aim to increase its water solubility, its carboxymethylated derivative, named CLIM205, was formed by the substitution of hydrogen atoms in LIM205 with one, two, and three carboxymethylate groups. FT-IR, UV, and ESI-MS analysis demonstrated that the carboxymethylate groups were conjugated onto LIM205. The lead detoxification activities of LIM205 and CLIM205 had also been investigated. In vivo test showed that both LIM205 and CLIM205 reduced the tissue lead concentration, enhanced lead excretion, and reversed lead-induced alterations in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) concentrations in mice, with CLIM205 showed better efficacy. The study indicates that LIM205 and CLIM205 have significant lead detoxification effect which will contribute to solve related problems.
在本研究中，一种名为LIM205的细胞内黑色素从拉氏菌YM205菌丝体中分离出来，并在葡聚糖G - 15柱上进行纯化。LIM205的分子量测定为522 Da，其分子式推测为CHNOS。根据傅里叶变换红外光谱（FT - IR）、氢核磁共振（H NMR）、碳核磁共振（C NMR）以及热解/气相色谱 - 质谱（pyrolysis/GC - MS）分析结果确定了LIM205可能的化学结构。为了提高其水溶性，通过用一个、两个和三个羧甲基基团取代LIM205中的氢原子，形成了其羧甲基化衍生物，命名为CLIM205。FT - IR、紫外光谱（UV）和电喷雾电离质谱（ESI - MS）分析表明羧甲基基团连接到了LIM205上。还研究了LIM205和CLIM205的铅解毒活性。体内试验表明，LIM205和CLIM205均可降低小鼠组织中的铅浓度，增强铅排泄，并逆转铅诱导的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH - Px）和丙二醛（MDA）浓度的改变，其中CLIM205表现出更好的效果。该研究表明，LIM205和CLIM205具有显著的铅解毒作用，这将有助于解决相关问题。
