Ye Ming, Chen Wu-Xi, Qiu Tao, Yuan Ru-Yue, Ye Ying-Wang, Cai Jing-Min
College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Food Chem. 2012 May 1;132(1):338-43. doi: 10.1016/j.foodchem.2011.10.087. Epub 2011 Oct 31.
A homogeneous extracellular polysaccharide of Lachnum YM261(LEPS-1) with a molecular weight of 21670Da was characterised. According to HPGPC, IR, periodate oxidation and Smith degradation, GC-MS and (1)H NMR analysis, the results indicated that LEPS-1 was a glucan linked by the β-(1→3)-d-pyran glycosidic bond. The effect of LEPS-1 on anti-ageing in d-gal model mice was also studied. It was found that LEPS-1 significantly increased the activities of antioxidant enzymes (i.e. SOD superoxide dismutase, CAT catalase, GSH-PX glutathione peroxidase) and decreased malondialdehyde (MDA) content in liver, brain and serum of d-gal model mice. These results showed that LEPS-1 had a strong anti-ageing activity.
对拉氏菌YM261的一种分子量为21670Da的均质细胞外多糖（LEPS-1）进行了表征。根据高效凝胶渗透色谱（HPGPC）、红外光谱（IR）、高碘酸盐氧化和史密斯降解、气相色谱-质谱联用（GC-MS）以及核磁共振氢谱（¹H NMR）分析，结果表明LEPS-1是一种由β-(1→3)-D-吡喃糖苷键连接的葡聚糖。还研究了LEPS-1对D-半乳糖模型小鼠的抗衰老作用。结果发现，LEPS-1显著提高了D-半乳糖模型小鼠肝脏、大脑和血清中抗氧化酶（即超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX））的活性，并降低了丙二醛（MDA）含量。这些结果表明LEPS-1具有较强的抗衰老活性。
