Lauková Andrea, Kandričáková Anna, Pleva Pavel, Buňková Leona, Ščerbová Jana
Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Department of Environmental Protection Engineering, Faculty of Technology, Tomáš Baťa University in Zlín, Vavrečkova 275, 762 72, Zlín, Czech Republic.
Folia Microbiol (Praha). 2017 May;62(3):229-235. doi: 10.1007/s12223-017-0492-0. Epub 2017 Jan 13.
In ostriches and pheasants, there is still limited information relating to staphylococci and their properties. Biogenic amines (BAs) are nitrogenous low-molecular-weight substances with biological functions in animals, plants and microorganisms. In this study, we focused on BA production by targeted faecal staphylococci from ostriches and pheasants and their sensitivity to lantibiotic bacteriocin gallidermin. Gallidermin belongs in a group of polycyclic proteinaceous antimicrobial substances. Thirty-six faecal staphylococci (24 strains from 140 ostriches, 12 from 60 pheasants) comprising different species were tested. Staphylococci from ostriches and pheasants did not produce tryptamine-TRYP, putrescine-PUT, cadaverine-CAD or histamine-HIS. Production of tyramine-TYM, phenylethylamine-PEA was high or very high (100-1000 mg/L). Production of spermine-SPM and spermidine-SPD by staphylococci was very low or low although in the case of staphylococci from pheasants medium production of SPM was found. Because of the risk posed by BAs for consumers, the control of BA-producing bacteria is important from the points of view not only of safety assessment of food-producing animals but also of human health safety. The sensitivity to gallidermin in biogenic amine-producing staphylococci from ostriches and pheasants detected here is the most promising indication for further application of gallidermin for veterinary purposes. The novelty of our study lies in testing the ability of faecal staphylococci from ostriches and pheasants to produce BAs and in their treatment with gallidermin which has so far not been tested in this way.
关于鸵鸟和雉鸡体内葡萄球菌及其特性的信息仍然有限。生物胺（BAs）是一类在动物、植物和微生物中具有生物功能的含氮低分子量物质。在本研究中，我们重点关注了鸵鸟和雉鸡粪便中目标葡萄球菌产生生物胺的情况以及它们对羊毛硫抗生素类细菌素加里道霉素的敏感性。加里道霉素属于多环蛋白质类抗菌物质。我们测试了36株不同种类的粪便葡萄球菌（其中24株来自140只鸵鸟，12株来自60只雉鸡）。鸵鸟和雉鸡体内的葡萄球菌不产生色胺 - TRYP、腐胺 - PUT、尸胺 - CAD或组胺 - HIS。酪胺 - TYM、苯乙胺 - PEA的产量较高或非常高（100 - 1000毫克/升）。葡萄球菌产生精胺 - SPM和亚精胺 - SPD的量非常低或较低，不过在来自雉鸡的葡萄球菌中发现有中等水平的SPM产生。由于生物胺对消费者构成风险，控制产生物胺的细菌不仅从食用动物的安全评估角度来看很重要，而且从人类健康安全角度来看也很重要。在此检测到的鸵鸟和雉鸡产生物胺葡萄球菌对加里道霉素的敏感性是加里道霉素进一步用于兽医用途的最有前景的指征。我们研究的新颖之处在于测试了鸵鸟和雉鸡粪便葡萄球菌产生物胺的能力以及用加里道霉素对其进行处理，而迄今为止尚未以这种方式进行过测试。
