Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05 110, Jablonna, Poland.
Probiotics Antimicrob Proteins. 2020 Mar;12(1):302-310. doi: 10.1007/s12602-019-9522-3.
Our current knowledge of microbiota in wild ruminants is limited. The goal of this study was to evaluate staphylococcal species in red and roe deer for various attributes (haemolysis, DNase, and urease activities; lactic acid and biofilm production; and antibiotic profile) and their susceptibility to gallidermin. Sixteen staphylococcal strains were identified from faeces of 21 free-living animals (9 adult female Cervus elaphus-red deer and 12 young female Capreolus capreolus-roe deer) sampled by the Polish colleagues in the Strzałowo Forest District, Piska Primaeval Forest. The variability in the species of staphylococci was determined. Seven species (Staphylococcus capitis, S. epidermidis, S. haemolyticus, S. hominis, S. pseudintermedius, S. vitulinus and S. warneri) and five clusters/groups of coagulase-negative staphylococci (CoNS) were identified. The strains were generally not haemolytic and Dnase negative; did not form biofilms or only produced low-grade biofilms; exhibited high levels of lactic acid; were urease positive; and were generally susceptible to antibiotics (only two strains were resistant to multiple antibiotics). However, all of the strains were susceptible to the lantibiotic bacteriocin gallidermin, with a minimal inhibitory concentration of 0.0156 μg (up to 6400 AU/ml in arbitrary units). This is the first study to perform a detailed study of the properties of CoNS from roe and red deer.
我们目前对野生动物肠道微生物群的了解有限。本研究旨在评估红鹿和狍粪便中的葡萄球菌属物种的各种特性（溶血、DNase 和脲酶活性；乳酸和生物膜生成；以及抗生素谱）及其对加替米星的敏感性。由波兰同事在皮斯卡原始森林的斯特拉沃洛森林区采集了 21 只自由生活的动物（9 只成年雌性马鹿和 12 只幼年雌性狍）的粪便，从中分离出 16 株葡萄球菌。确定了葡萄球菌属物种的变异性。鉴定出了 7 个种（头葡萄球菌、表皮葡萄球菌、溶血葡萄球菌、人葡萄球菌、中间葡萄球菌、魏氏葡萄球菌和华纳葡萄球菌）和 5 个凝固酶阴性葡萄球菌（CoNS）聚类群。这些菌株通常不溶血且无 Dnase 活性；不形成生物膜或仅产生低水平的生物膜；产生高水平的乳酸；脲酶阳性；通常对抗生素敏感（只有两株对多种抗生素耐药）。然而，所有菌株均对抑菌肽加替米星敏感，最小抑菌浓度为 0.0156μg（在任意单位中高达 6400AU/ml）。这是首次对来自狍和马鹿的 CoNS 特性进行详细研究的研究。
