Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05 110, Jablonna, Poland.
Folia Microbiol (Praha). 2019 Nov;64(6):719-726. doi: 10.1007/s12223-019-00684-5. Epub 2019 Jan 31.
The intestinal microbiota has enormous impact on the health and performance of horses. Staphylococci belong in the phylum Firmicutes, and their occurrence, especially of methicillin-resistant strains and species, has been reported in horses previously. Moreover, biofilm formation is one of the virulence factors; it has been not completely studied in fecal coagulase-negative staphylococci (CoNS) from horses. Therefore, this study was focused on biofilm formation by various species of fecal CoNS from horses because it has been never reported before. In addition, their antibiotic profile was tested. Horses (42) of various breeds from Slovakia/Poland were sampled. Variability in the species of CoNS was detected in feces of horses. Thirty-two strains were identified by using the MALDI-TOF system and classified into nine species and three subspecies of CoNS: Staphylococcus capitis, S. cohnii subsp. cohnii, S. cohnii subsp. urealyticus, S. cohnii subsp. casei, S. epidermidis, S. haemolyticus, S. pasteuri, S. sciuri, S. vitulinus, S. warneri, and S. xylosus. The most frequent species was S. vitulinus. Twenty-two strains showed high biofilm production; 10 strains showed low-grade biofilm production. The highest biofilm formation was measured in the species S. xylosus. Eleven strains (of 32) were methicillin-resistant; the others were susceptible to methicillin.
肠道微生物群对马的健康和性能有巨大影响。葡萄球菌属于厚壁菌门，以前曾有报道称它们在马中存在，特别是耐甲氧西林的菌株和物种。此外，生物膜形成是一种毒力因素；在来自马的粪便凝固酶阴性葡萄球菌（CoNS）中尚未完全研究。因此，本研究集中于粪便 CoNS 的各种物种的生物膜形成，因为以前从未有过报道。此外，还测试了它们的抗生素特性。从斯洛伐克/波兰采集了来自各种品种的 42 匹马。在马的粪便中检测到 CoNS 物种的变异性。使用 MALDI-TOF 系统鉴定了 32 株，并将其分为 9 个种和 3 个亚种的 CoNS：头葡萄球菌、Cohnii 亚种 Cohnii、Cohnii 亚种脲解葡萄球菌、Cohnii 亚种乳球菌、表皮葡萄球菌、溶血葡萄球菌、巴氏葡萄球菌、松鼠葡萄球菌、维鲁葡萄球菌、沃氏葡萄球菌和木糖葡萄球菌。最常见的物种是维鲁葡萄球菌。22 株表现出高生物膜产生；10 株表现出低生物膜产生。在木糖葡萄球菌种中测量到最高的生物膜形成。11 株（32 株中的）耐甲氧西林；其余对甲氧西林敏感。
