Shen Xianjuan, Zhang Yan, Wu Xian, Guo Yuehua, Shi Wei, Qi Jing, Cong Hui, Wang Xudong, Wu Xinhua, Ju Shaoqing
Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
Cancer Biomark. 2017;18(3):257-263. doi: 10.3233/CBM-160158.
The purpose of this study was to explore serum PCAT-1 expression in multiple myeloma (MM) and examine the potential usefulness of this molecule as a biomarker for diagnosis in MM.
Serum samples were collected from 60 newly diagnosed untreated MM patients, and 48 healthy controls. Serum PCAT-1 expression levels were detected by RT-qPCR. In addition, correlations between the relative expression of serum PCAT-1 and the concentrations of lactic acid dehydrogenase (LDH), β2M, λ light chain and κ light chain were assessed.
It was found that the relative expression of serum PCAT-1 in MM patients (81.02 ± 136.9) was higher than that in healthy controls (3.17 ± 5.75) (U= 307.0, P< 0.0001) and was significantly correlated with β2M concentration (r= 0.461, P= 0.0002), but not with LDH, κ light and λ light chain concentration (r= 0.061, P= 0.641; r= 0.007, P= 0.956; r=-0.090, P= 0.499 respectively). Additionally, it was significantly correlated with different isotype of MM (H= 7.464, P= 0.024). The AUC of the ROC curve of serum PCAT-1 was 0.892 (95% CI 0.833-0.950), which was higher than other markers. Combining PCAT-1 and β2M together, the sensitivity was highest compared with other markers alone, or combined.
The expression levels of serum PCAT-1 in MM patients were significantly higher than that in healthy controls, suggesting that it may be useful in the auxiliary diagnosis of MM.
本研究旨在探讨多发性骨髓瘤（MM）患者血清中PCAT-1的表达情况，并检验该分子作为MM诊断生物标志物的潜在用途。
收集60例新诊断的未经治疗的MM患者和48例健康对照者的血清样本。采用RT-qPCR检测血清中PCAT-1的表达水平。此外，评估血清PCAT-1相对表达量与乳酸脱氢酶（LDH）、β2M、λ轻链和κ轻链浓度之间的相关性。
发现MM患者血清PCAT-1的相对表达量（81.02±136.9）高于健康对照者（3.17±5.75）（U=307.0，P<0.0001），且与β2M浓度显著相关（r=0.461，P=0.0002），但与LDH、κ轻链和λ轻链浓度无关（r分别为0.061，P=0.641；r=0.007，P=0.956；r=-0.090，P=0.499）。此外，它与MM的不同亚型显著相关（H=7.464，P=0.024）。血清PCAT-1的ROC曲线下面积为0.892（95%CI 0.833-0.950），高于其他标志物。将PCAT-1和β2M联合使用时，与其他单独或联合使用的标志物相比，敏感性最高。
MM患者血清PCAT-1的表达水平显著高于健康对照者，提示其可能有助于MM的辅助诊断。
