Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Department of Laboratory Medicine, Affiliated Hospital of Nantong University, No 20, Xisi Road, Nantong, 226001, China.
Ann Hematol. 2020 Sep;99(9):2159-2171. doi: 10.1007/s00277-020-04161-9. Epub 2020 Jun 26.
Increasing knowledge of long non-coding RNAs (lncRNAs) has shown that they can be used as circulating tumor markers. Also, considerable evidences have revealed that lncRNAs have important roles in tumor diagnosis and prognosis. The lncRNA CCAT2 has manifested its carcinogenic effect in a variety of tumors, but the serum expression level and clinical value in multiple myeloma (MM) remain to be explored. In our study, the expression of lncRNA CCAT2 is upregulated in the serum and bone marrow of MM patients by using quantitative real-time polymerase chain reaction (qRT-PCR). The high expression level of CCAT2 in the serum of MM patients correlated with International Scoring System (ISS) stages, renal dysfunction, serum β-microglobulin (β-MG) concentration, and light chain (κ and λ) concentrations. Area under the curve (AUC) of CCAT2 in serum is 0.899. Besides, the sensitivity and specificity were 85.80% and 83%, respectively. Furthermore, combination of CCAT2, IgA, HGB, and β-MG significantly improved the MM diagnostic sensitivity and AUC. Here, our present investigation indicates that serum circulating CCAT2 may serve as a potential tumor marker for diagnosis and prognosis of MM.
越来越多的长链非编码 RNA（lncRNA）知识表明，它们可以作为循环肿瘤标志物。此外，大量证据表明 lncRNA 在肿瘤诊断和预后中具有重要作用。lncRNA CCAT2 在多种肿瘤中表现出致癌作用，但在多发性骨髓瘤（MM）中的血清表达水平和临床价值仍有待探索。在我们的研究中，通过实时定量聚合酶链反应（qRT-PCR）发现 MM 患者血清和骨髓中的 lncRNA CCAT2 表达上调。MM 患者血清中 CCAT2 的高表达水平与国际分期系统（ISS）分期、肾功能不全、血清β-微球蛋白（β-MG）浓度和轻链（κ 和 λ）浓度相关。CCAT2 在血清中的曲线下面积（AUC）为 0.899。此外，CCAT2 的灵敏度和特异性分别为 85.80%和 83%。此外，CCAT2、IgA、HGB 和β-MG 的联合检测显著提高了 MM 的诊断敏感性和 AUC。本研究表明，血清循环 CCAT2 可能作为 MM 诊断和预后的潜在肿瘤标志物。
