Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
J Microbiol Biotechnol. 2020 Apr 28;30(4):533-539. doi: 10.4014/jmb.1908.08054.
A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of and , which utilize protein as carbon source. Experimental data of changes in peptone concentration and 16S rRNA gene copy numbers of and were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squared analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate (), half saturation concentration (), growth yield (), and decay coefficient () of and were 0.73 ± 0.05 and 1.35 ± 0.32 h, 6.07 ± 1.52 and 5.67 ± 1.53 g/L, 2.25 ± 0.75 × 10 and 7.92 ± 3.71 × 10 copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h, respectively. The theoretical specific growth rate of always exceeded than that of at peptone concentration higher than 3.62 g/L. When the influent peptone concentration was 5.0 g/L, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 10 copies/mL at 4 hours HRT, which indicates 67.1% of the initial population reduction, but the wash out occurred at 1.9 and 3.2 hours HRTs. The 16S rRNA gene copy numbers of gradually decreased to steady values ranging from 1.1 × 10 to 2.9 × 10 copies/mL. species was predicted to wash out at an HRT of 1.6 h.
采用实时荧光定量聚合酶链反应（QPCR）来估算 和 利用蛋白质作为碳源的生物动力学系数。将 和 的 16S rRNA 基因拷贝数和蛋白胨浓度变化的实验数据拟合到模型中。采用四阶龙格-库塔逼近法和非线性最小二乘法分析来求解常微分方程，以估计生物动力学系数。 和 的最大比生长速率（）、半饱和浓度（）、生长产率（）和衰减系数（）分别为 0.73 ± 0.05 和 1.35 ± 0.32 h、6.07 ± 1.52 和 5.67 ± 1.53 g/L、2.25 ± 0.75×10 和 7.92 ± 3.71×10 拷贝/g、0.002 ± 0.003 和 0.002 ± 0.001 h。在蛋白胨浓度高于 3.62 g/L 时， 的理论比生长速率始终高于 的比生长速率。当进水蛋白胨浓度为 5.0 g/L 时，4 小时 HRT 时 C.cadaveris 的浓度逐渐降至稳定值 2.9×10 拷贝/mL，表明初始种群减少了 67.1%，但在 1.9 和 3.2 小时 HRT 时发生了洗脱。 的 16S rRNA 基因拷贝数逐渐降至 1.1×10 至 2.9×10 拷贝/mL 的稳定值。预测 在 HRT 为 1.6 h 时将被洗出。
