Beshiru Abeni, Igbinosa Isoken H, Omeje Faith I, Ogofure Abraham G, Eyong Martin M, Igbinosa Etinosa O
Applied Microbial Processes & Environmental Health Research Group, Department of Microbiology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154, Benin City 300001, Nigeria; Department of Microbiology and Biotechnology, College of Natural and Applied Sciences, Western Delta University, Private Mail Bag 10, Oghara, Delta State, Nigeria.
Department of Environmental Management and Toxicology, Faculty of Life Sciences, University of Benin, Private Mail Bag 1154, Benin City 300001, Nigeria; SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
Microb Pathog. 2017 Mar;104:90-96. doi: 10.1016/j.micpath.2017.01.020. Epub 2017 Jan 11.
The continuous misuse of antimicrobials in food animals both orally and subcutaneously as therapeutic and prophylactic agents to bacterial infections could be detrimental and contribute to the dissemination of resistant clones in livestock production. The present study was carried out to determine the antibiogram and virulence gene characteristics of Enterococcus species from pig farms. A total of 300 faecal samples were obtained from two pig farms in Benin City between February and July 2016. Standard culture-based and polymerase chain reaction (PCR) assay were adopted in the detection and characterization of the Enterococcus species. Antimicrobial susceptibility profile was determined using disc diffusion method. A total of 268 enterococci isolates were recovered from both farms investigated. In Farm A, 94/95 (99%) of E. faecalis isolates were resistant to clindamycin; while 23/25 (92%) of E. faecium isolates were resistant to clindamycin. In farm B, all E. faecalis isolates 119/119 (100%) were resistant to clindamycin; while 26/29 (90%) of E. faecium isolates were resistant to clindamycin. Virulence gene detected in the enterococci isolates includes aggregation (asa1) [Farm A (E. faecalis 66%, E. faecium 76%), Farm B (E. faecalis 71%, E. faecium 13%)] and others. Multidrug resistant profile of the isolates revealed that 17/95 (18%) of E. faecalis and 3/25 (12%) of E. faecium isolates from Farm A as well as, 16/119 (14%) of E. faecalis and 5/29 (17%) of E. faecium isolates from Farm B were resistant to CLI, PEN, ERY, GEN, TET, MEM, KAN, and PTZ. The high level of resistance observed in the study and their virulence gene signatures, calls for effective environmental monitoring to circumvent the environmental dissemination of resistant pathogenic clones. Thus environmental hygiene should be provided to food animals to prevent the proliferation and spread of resistant bacteria.
在食用动物中持续将抗菌药物作为治疗和预防细菌感染的药物口服和皮下滥用可能是有害的，并会导致耐药克隆在畜牧生产中的传播。本研究旨在确定来自养猪场的肠球菌的抗菌谱和毒力基因特征。2016年2月至7月期间，从贝宁城的两个养猪场共采集了300份粪便样本。采用基于标准培养的方法和聚合酶链反应（PCR）检测及鉴定肠球菌。使用纸片扩散法测定抗菌药物敏感性。从两个调查的农场共分离出268株肠球菌。在农场A，94/95（99%）的粪肠球菌分离株对克林霉素耐药；而23/25（92%）的屎肠球菌分离株对克林霉素耐药。在农场B，所有119/119（100%）的粪肠球菌分离株对克林霉素耐药；而26/29（90%）的屎肠球菌分离株对克林霉素耐药。在肠球菌分离株中检测到的毒力基因包括聚集（asa1）[农场A（粪肠球菌66%，屎肠球菌76%），农场B（粪肠球菌7
